摘要
Abstract
AIM: To investigate the influence of Notch1 signaling on expression of T-bet,Th1- and Th2-type cytokines and cytotoxic Tlymphocyte (CTL) cytotoxicity of peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis B, and analyze the possible function of Notch1 signaling on regulating the activation of T cellular immunity.METH ODS: PBMC of patients with chronic hepatitis B were divided into test group, negative control group and blank control group, respectively was given anti-Notch1, IgG2b and RPMI-1640.The expression of T-bet mRNA was examined by real-time PCR, Th1-type cytokines IFN-γ, IL-2,Th2-type cytokines IL-4, IL-10 were assayed by enzyme-linked immunosorbent assay (ELISA) and CTL cytotoxicity was measured by Cytotoxicity Detection Assay (LDH).RESULTS: After Notch1 signaling of PBMC was blocked in chronic hepatitis B patients, expression of T-bet mRNA and the production of Th1-type cytokines IFN-γ,IL-2 were enhanced while Th2-type cytokines IL-4,IL-10 were decreased, and CTL cytotoxicity was enhanced, conipared with the blank control groups (P＜0.01).CONCLUSION: After blocking of Notch1 signaling of PBMC of chronic hepatitis B patients, the expression of Tbet mRNA and the production of Th1-type cytokines IFN-γ, IL-2 were increased, while Th2type cytokines IL-4, IL-10 were decreased and CTL cytotoxicity was enhanced.It suggested that blockage of Notch1 signaling could activate T cellular immunity of patients with chronic hepatitis B, Notch1 signaling might contribute to immune tolerance of patients with chronic hepatitis B, perhaps related with T-bet.关键词
慢性乙肝/Notchl/T细胞免疫/T-bet/CTL分类
医药卫生
