中国临床药理学与治疗学2011，Vol.16Issue(2)：121-125,5.
脑源性神经营养因子对大鼠海马神经元GABAA受体效应的影响
Effect of brain-derived neurotropic factor on GABAA receptor function in hippocampal neurons
摘要
Abstract
AIM: To investigate the effect of brain-derived neurotrophic factor (BDNF) on GABAA receptor function in cultured hippocampal neurons of rats. METHODS: Primary cultured hippocampal neurons were treated with BDNF (100, 300, or 1000 ng/L) for 20 min,and the intracellular calcium concentration was measured using flow cytometry 5 min after GABA application. GABAA-gated currents were measured using whole cell patch-clamp recordings, the potential was held at -60 mV, BDNF (100, 300, or 1000 ng/L) was applied to the neurons for 2 min, then GABA 100 μmol/L was applied for 5 s, and GABAA-gated currents were recorded. In another experiment, the potential was clamped from 100 mV to 0 mV with increment of 10 mV, GABAA-gated currents were recorded under the influence of BDNF, and the reversal potential of GABAA-gated currents was achieved. RESULTS: After treatment with BDNF 100 ng/L or 300 ng/L, the GABA- induced intracellular calcium concentrations in pyramidal cells were not changed, GABAA-gated currents at -60 mV of the held potential and the reversal potential were not affected. But under the influence of BDNF 1000 ng/L, GABAinduced intracellular calcium concentration in pyramidal cells was increased significantly compared with baseline (P＜0.05), GABAA-gated currents were transformed to outward currents at -60 mV of the held pontential, and the reversal potential of GABAA-gated currents were left-shifted from 0 mV in control to -93 mV.CONCLUSION: BDNF with high concentration can make the reversal potential of GABAA-gated currents shifted leftward, and causes GABA to induce an excitatory effect through GABAA receptor.关键词
脑源性神经营养因子/海马神经元/GABAA受体分类
医药卫生引用本文复制引用
刘红亮,戴体俊..脑源性神经营养因子对大鼠海马神经元GABAA受体效应的影响[J].中国临床药理学与治疗学,2011,16(2):121-125,5.基金项目
国家自然科学基金资助课题(30471657) （30471657）
