畜牧兽医学报2011，Vol.42Issue(3)：349-355,7.
鸡Perilipin1抗血清制备及组织表达特性分析
Preparation of Antiserums against Chicken Perilipin1 and Tissue Expression Analyses of Perilipin1
摘要
Abstract
The aim of this study was to analyze the expression characteristics of chicken Perilipinl in different tissues and the function of Perilipinl in lipid metabolism by preparing the antiserum against chicken Perilipinl. Chicken Perilipinl gene was amplified by RT-PCR and inserted into pET-28a vector. The recombinant His/Perilipinl was expressed in E. coli with IPTG induction and renatured by Protein Refolding Kit. The renatured His/Perilipinl was used as immunogen to immunize the rabbit for preparing polyclonal antibody. The titer and specificity of this antibody were detected by ELISA and Western blot, and the expression characteristics of chicken Perilipinl in different tissues was analyzed by Western blot. ELISA and Western blot results showed that the high titer and specificity of the antiserum against Perilipinl was obtained. The tissue expression characterization analysis by Western blot showed that Perilipinl only expressed in chicken adipose tissue, but no signal was detected in liver, duodenum, breast muscle, leg muscle, giz zard, heart, spleen and kidney. Furthermore, the Perilipinl expression characteristics between fat and lean broiler lines were also detected, and the results showed that Perilipinl expressed significantly higher in fat line broilers adipose tissue than that in lcan ones (P＜O. 05). These results of this study suggest that there is a close relationship between the expression of Perilipinl and the deposition of abdomen adipose in fat and lean broiler lines.关键词
鸡/脂滴包被蛋白/抗血清/表达特性分类
农业科技引用本文复制引用
王彦博,王宁,王丽,王宇祥,李玉茂,李辉..鸡Perilipin1抗血清制备及组织表达特性分析[J].畜牧兽医学报,2011,42(3):349-355,7.基金项目
国家重点基础研究发展计划(973计划)项目(2006CB102105) （973计划）
国家高技术研究发展计划(863)项目(2006AA10A120) （863）
国家重点基础研究发展计划(973计划)项目(2009CB941604) （973计划）
