畜牧兽医学报2011，Vol.42Issue(3)：363-368,6.
牛SCD1基因C878T位点遗传多态性与中国西门塔尔牛部分脂肪相关性状的效应分析
Genetic Variation at C878T in Bovine SCD1 Gene and Its Association with Some Fat Traits in Chinese Simmetal Cattle
摘要
Abstract
This experiment was conducted to study the effect of SCD1 gene. To explore the effect of genetic variance in SCD1 gene on carcass and meat traits,682 cattle were selected randomly,including Chinese Simmetal cattle ( Simme) , Leiqiong cattle (LQ) , Yunnan Gaofeng cattle (GF) ,BMY cattle (BMY) , Minnan cattle (MN), Luxi cattle ( LX) , Bohai Black cattle (BH) and Holstein of South Chinese ( Hols. ) ,the polymorphism of specific fragment of SCD1 gene was analyzed by PCR-SSCP technique. A nucleotide mutation had been found at site 878, leading to an amino acid change (Glycine to Valine) at site 293,it was C878T. Three genotypes (CC,CT and TT) had been found at this site,and the frequency of genotype TT was highest in Chinese Simmetal population with the value of 0. 114, and lower in Luxi cattle , Bohai Black cattle and Holstein with the value of 0. 050,0. 063 and 0. 040,respectively. But genotype TT had not been found in the four tropic populations including Leiqiong cattle, Yunnan Gaofeng cattle , BMY cattle and Minnan cattle. The individuals with CC genotype of SCD1 showed a higher content of IMF and Mesentery fat weight traits ( P＜0. 05) , but lower value of backfat thickness trait ( P＜0. 01) , compared to the individuals with TT genotype. There was no significant difference among different genotypes with other traits. The results indicate that the C878T mutation of SCD1 gene has a great genetic effect on carcass and meat traits and could be a useful genetic marker for Chinese beef breeding.关键词
SCD1基因/屠宰性状/肉质性状/SNP分类
农业科技引用本文复制引用
武秀香,施雪奎,常玲玲,杨章平,李俊雅,毛永江,高会江..牛SCD1基因C878T位点遗传多态性与中国西门塔尔牛部分脂肪相关性状的效应分析[J].畜牧兽医学报,2011,42(3):363-368,6.基金项目
国家科技支撑计划(2008BADB2B03-06) （2008BADB2B03-06）
863重点项目(2008AA101010) （2008AA101010）
