畜牧兽医学报2011，Vol.42Issue(3)：402-408,7.
鸡β-防御素-1对鸡传染性法氏囊病病毒VP2基因DNA疫苗的免疫佐剂作用
The Adjuvant Effects of Chicken β-defensin-1 on Infectious Bursal Disease Virus VP2 Gene DNA Vaccine
摘要
Abstract
The aim of present study was to investigate the adjuvant effects of chicken β-defensin-1 (also named avian β-defensin-1, AvBD1) on DNA vaccine encoding chicken infectious bursal disease virus (IBDV) VP2 gene. The eukaryotic expression vectors pcDNA3.1 (+)-AvBD1 and pcDNA3. 1(+)-VP2 were constructed. Five groups of 14-day-old chickens were intramuscularly injected with PBS, empty vector pcDNA3. 1(+), pcDNA3. 1(+)-VP2, a mixtures of pcDNA3. 1 (+)-AvBD1 and pcDNA3.1 (+)-VP2, and IBD attenuated vaccine, respectively. Serum of chicken in each group was collected from the peripheral blood at different time post inoculation,and VP2-specific antibody was measured by ELISA. Content of CD3+ , CD4+ and CD8+ T-lymphocytes in each group of chickens were tested by cytospectrometrv. Results showed that VP2specific antibody level of group immunized with a mixtures of pcDNA3. 1(+)-AvBD1 and pcDNA3.1(+)-VP2 was the highest among all the groups. The VP2-specific antibody lev.el of group immunized with pcDNA3. 1(+)-VP2, as well as group immunized with IBD attenuated vaccine,was significantly higher than that of the group immunized with PBS and empty vector pcDNA3. 1 (+). The percentages of CD3+ , CD4+ and CD8+ T-cell subtypes between groups of pcDNA3.1 (+)-VP2 and pcDNA3. 1 (+)-VP2 combination with pcDNA3.1 (+)-AvBDI was significantly different (P＜0. 05), the latter was higher, at 7 days post boost. The protection from IBD challenged by immunized chickens with DNA vaccines encoding IBDV VP2 gene alone was lower than that by immunized with a mixtures of pcDNA3. 1(+)-AvBD1 and pcDNA3. 1(+)-VP2. The results indicated that AvBD1 can be used as an adjuvant to enhance the IBDV VP2 DNA vaccine immunity.关键词
鸡β-防御素-1(AvBD1)/免疫佐剂/IBDV/DNA疫苗分类
农业科技引用本文复制引用
张辉华,杨小梅,谢青梅,马静云,罗艳娜,曹永长,毕英佐..鸡β-防御素-1对鸡传染性法氏囊病病毒VP2基因DNA疫苗的免疫佐剂作用[J].畜牧兽医学报,2011,42(3):402-408,7.基金项目
广东省自然科学基金项目(5300444) （5300444）
广东省科技厅农业科技攻关项目(2006B20101015) （2006B20101015）
