畜牧兽医学报2011，Vol.42Issue(3)：437-441,5.
应用MGB荧光探针技术确定猪KIT基因拷贝重复数
Detecting Copy Number Variations in Porcine KIT by TaqMan-MGB Probe
摘要
Abstract
The main objective of the present study was to establish a simple, rapid method for detecting copy number variations (CNVs) in porcine KIT gene. TaqMan-MGB probes and primers were designed according to sequence of exon 2 of KIT gene, and a real-time fluorescence quantitative PCR procedure was established for the CNVs detection. The results showed that amplification curves of KIT obtained by TaqMan-MGB probe were a series of parallel curves corresponding to 2-fold serial dilutions of DNA samples, Ct values between groups were significantly different (P＜0. 001) , and the coefficient-of-variations were low (from 0. 12％ to 0. 26％). The amplification effiaencies of the KIT and ESR were approximately equal. The CNVs in KIT of 50 pigs were estimated by cluster analysis, assigned to 2, 3, 4, 5 or 6 copies, respectively. The real-time quantitative PCR using TaqMan-MGB probe is a simple, rapid method with high resolution and stability to measure CNVs in KIT, and it could be carried out in common laboratories.关键词
猪/显性/KIT位点/拷贝数/MGB探针/荧光定量PCR分类
农业科技引用本文复制引用
王韬,唐辉,曾勇庆,李晓宁,闫超,董林松,于希江..应用MGB荧光探针技术确定猪KIT基因拷贝重复数[J].畜牧兽医学报,2011,42(3):437-441,5.基金项目
山东省中青年科学家奖励基金(2008BS07009) （2008BS07009）
山东省科技攻关(2009GG10009036) （2009GG10009036）
国家863重大专项(2008101002) （2008101002）
