畜牧兽医学报2011，Vol.42Issue(3)：442-447,6.
序贯培养液G1/G2对山羊核移植胚胎发育和质量的影响
Effect of Sequential Media G1/G2 on Development and Quality of Goat SCNT Embryos
摘要
Abstract
The aims of this study were to investigate the effects of sequential media G1/G2 and modified synthetic oviductal fluid (mSOF) culture systems on developmental competence, cell apoptosis and the pregnant rate of goat somatic cell nuclear transfer (SCNT) embryos. Goat NT embryos were cultured as following: (1) 72 h in G1 then 120 h in G2 (group G1G2) , both the culture media supplemented with 0. 8％ BSA (bovine serum albumin) ; (2) 72 h in mSOF supplemented with 0. 8％ BSA then in mSOF supplemented with 10％ FBS (fetal bovine serum) for 120 h (group mSOF-FBS). The results indicated that there was no significant difference between the sequential media G1/G2 and mSOF-FBS in blastocyst rates ((27. 7±3. 1) ％ vs (25. 3±1. 0) ％,P ＞0. 05). Both of total cell number of blastocysts and apoptotic nuclei in blastocyst from G1/G2 were significantly lower than that in mSOF-FBS ( (93. 2±4. 5) vs (109. 1± 6. 2) and (4. 9 ±0. 2) ％ vs(11. 3±0. 1) ％ , respectively, P＜0. 05 ). However, the pregnant rates on Day 30 in group G1/GZ were higher than those in group mSOF-FBS(21. 4％ vs 8. 0％, P＜O. 05 ). In conclusion, compared to mSOF-FBS, the sequential media G1/G2 could better support the development of goat somatic cell nuclear transfer embryos.关键词
山羊/胚胎培养/体细胞核移植/凋亡分类
农业科技引用本文复制引用
刘军,郑聪颖,李长雷,杜珊,李亮亮,马保华,张涌..序贯培养液G1/G2对山羊核移植胚胎发育和质量的影响[J].畜牧兽医学报,2011,42(3):442-447,6.基金项目
国家转基因生物新品种培育科技重大专项(2008ZX08007-004)资助 （2008ZX08007-004）
