军事医学2011，Vol.35Issue(5)：333-337,5.
安多霖对微波辐射致大鼠海马细胞色素C氧化酶变化的影响
Effects of AduoLa Fuzhenglin on changes of cytochrome c oxidase in rats′ hippocampus after microwave exposure
摘要
Abstract
Objective To investigate the effect of a herb compound AduoLa Fuzhenglin ( ADL ) on the gene cytochrome c oxidase ( COX ) change of the mitochondrial respiratory chain in rats' hippocampus after microwave exposure. Methods One hundred Wistar male rats were exposed to microwave at the average power density of 30 mW/cm2for 15 min,3 g/( kg .d ) ADL was administrated for 14 days before or after radiation. Rats were sacrificed at 6 h and 14 d after exposure. The activity of COX was determined by colorimetry; real time polymerase chain reaction was used to study the expression of COX Ⅰ and Ⅳ mRNA; the COX Ⅰ protein was detected by Western blot and image analysis. Results The level of COX activity, COX Ⅰ and Ⅳ mRNA and COX protein was decreased obviously in the radiated control group ( P ＜0. 05 or P ＜0. 01 ) at 6 h and in the prophylaxis and treatment groups after drug withdrawal. The activity and gene expression of COX in the ADL prophylaxis group were higher than those in the radiated control group, and were not significant different from the normal control. Compared with the radiated control group, those in the ADL treatment group were significantly increased ( P ＜ 0. 05 ), and almost returned to normal. Conclusion Administration of 3 g/( kg . d ) ADL could lessen the decrease in the COX expression level of the mitochondrial respiratory chain in rats' hippocampus after microwave exposure, and with better effect on ADL treatment group.关键词
安多霖/微波辐射/大鼠/海马/COX分类
医药卫生引用本文复制引用
熊璐,徐新萍,王晓民,刘曙晨,彭瑞云,高亚兵,王水明,王丽峰,董霁,赵黎,李杨,左红艳..安多霖对微波辐射致大鼠海马细胞色素C氧化酶变化的影响[J].军事医学,2011,35(5):333-337,5.基金项目
国家科技重大专项(2008ZXJ09004-019) （2008ZXJ09004-019）
