中国临床医学2011，Vol.18Issue(2)：136-138,3.
胡椒属药材核糖体DNA的ITS序列分析及其分子鉴定
Identification of Medicinal Plants from Piper L.Based on rDNA ITS Sequences
摘要
Abstract
Objective: Study on the applicability of rDNA ITS sequence in identification of medicinal plants from Piper L. and its related species. Methods: The ITS regions of these species were amplified by PCR and sequenced, and their sequences were analyzed by DN Assist Version 2. 2. Results: Homologous alignment indicated that the consanguinity of the ITS sequences between Genus Piper and outgroups was lower than 67.0％, and that within genus Piper was higher than 82.0％. For the same species, the consanguinity was higher than 99.0％. Conclusions: The ITS sequences may serve as reliable molecular markers for identification of Genus Piper and its related species.关键词
胡椒属/ITS序列鉴定分类
农业科技引用本文复制引用
蔡诚诚,杨志业,谢晖,潘胜利..胡椒属药材核糖体DNA的ITS序列分析及其分子鉴定[J].中国临床医学,2011,18(2):136-138,3.基金项目
国家科技部重大专项"重大新药创制"(编号:2009ZX09103-075) （编号:2009ZX09103-075）
上海市科委中药现代化专项(编号:08DZ1971203) （编号:08DZ1971203）
