中国美容医学2011，Vol.20Issue(3)：430-433,4.
肝细胞生长因子对大鼠背部超长任意皮瓣存活率影响的实验研究
Experimental study on effect of hepatocyte growth factor on the survival rate of random ultralong skin flap on the rat back
王家瑞 1刘达恩 1农庆文 1梁坤兰 1李顺堂 1李开通1
作者信息
- 1. 广西医科大学第一附属医院烧伤整形科,广西,南宁,530021
- 折叠
摘要
Abstract
Objective To investigate the angiogenesis function of hepatocyte growth factor (HGF) to random ultra-long skin flap on the rat back and its effect on the survival rate of the skin flap. Methods Making a 8.0cmx2.0cm animal model from a random ultra-long skin flap on the rat back, injected directly with 50ng/ml HGF solution, after 7 days observing the color of the skin flap, texture, capillary return, necrotic area, bleeding condition of the cut flap etc;Calculating the survival area of the flap; After immunity staining, counting blood capillary with a 400 optical microscope.Making results through comparing the disparity between the before-experimental flap and that of after-experiment.,And also comparing with the saline control group. Results Comparing the survival rate (81.45％±2.74％) of the HGFskin flap with the control group (42.82％±7.03％), the differences are statistically significant (P ＜0.05); before experiment,The quantity of capillary of the experimental group and the control group are respectively (12.70±1.35) and (12.31±1.22),the difference between the two is of statistic significance (P＞0.05); After experiment, the quantity of capillary of the experimental group and the control group are respectively (39.67±4.83) and (21.50±1.87), The difference between the two is of statistic significance (P＜0.05); After experiment, the number of capillary of the experimental group is higher than that before experiment, the difference is statistically significant (P＜0.05). Conclusion HGF can promote the formation of the vascular endothelial cells in the flap and improve the survival rate of skin flaps.关键词
肝细胞生长因子/超长任意皮瓣/血管内皮细胞/存活率Key words
hepatocyte growth factor (HGF)/ random ultra-long skin flap/ vascular endothelial cells/ survival分类
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王家瑞,刘达恩,农庆文,梁坤兰,李顺堂,李开通..肝细胞生长因子对大鼠背部超长任意皮瓣存活率影响的实验研究[J].中国美容医学,2011,20(3):430-433,4.
