山东医药2011，Vol.51Issue(10)：17-18,2.
人胆固醇酯水解酶重组腺病毒载体的构建
Construction of human CEH recombinant adenovirus vector
摘要
Abstract
Objective To construct hCEH recombinant replication deficient adenovirus vector pAd-hCEH amplify in HEK293 cells.Methods hCEH gene was cloned into shuttle vector pAdTrack-CMV to generate a recombinant plasmid pAdTrack-CMV-hCEH, and the resultant plasmid was linearized and was subsequently homogenous recombination with pAdeasy-1 in E.coli BJ5183 cells.Recombinants were screened and were identified by observation of green fluorescent protein (GFP) and PCR.The recombinant plasmid was transfected into HEK293 to package the adenovirus, and the hCEH recombinant adenovirus was observed by fluorescent microscope.Results The hCEH recombinant adenoviral vector was constructed successfully, which was confirmed by restriction enzyme and GFP expression.High titer recombinant adenoviral vector was constructed.Conclusion The hCEH recombinant adenovirus is constructed successfully.关键词
胆固醇酯水解酶/腺病毒/质粒/人胚肾细胞/腺病毒载体系统Key words
cholesterol ester by drolase/ adenovirus/ plasmid/ human embryonic kidney cell/ adenovirus vector system分类
医药卫生引用本文复制引用
张兴海,罗俊生,霍晓川,顾立学,关宁,冯旭..人胆固醇酯水解酶重组腺病毒载体的构建[J].山东医药,2011,51(10):17-18,2.基金项目
国家自然科学基金资助项目(30971023) （30971023）
辽宁省自然科学基金项目(C090301) （C090301）
辽宁省教育厅高等学校科研项目计划(2009A481). （2009A481）
