山东医药2011，Vol.51Issue(14)：6-7,2.
5-氮杂-2'-脱氧胞苷对胃癌AGS细胞CHFR基因去甲基化作用及意义
Demethylation of CHFR gene in gastric cancer cell line AGS by 5-Aza-CdR and its clinical applications
邱庆安 1吕云福 1陈一明 1林友刚 1伍海鹰 1刘宁1
作者信息
摘要
Abstract
Objective To explore demethylation effect of 5-Aza-2'deoxycytidine (5-Aza-CdR)in gastric cancer cell line AGS and study its clinical applications.Methods Bisulfite sequencing PCR (BSP) and reverse transcriptase PCR ( RT-PCR ) were used to detect CHFR gene promoter's methylation status and its mRNA expression pre and post 5-Aza-CdR treatment in gastric cancer cell line AGS respectively.Results CHFR gene promoter displayed hypermethylated in gastric cancer cell line AGS (methylation rate≥60％ ), while it was low after 5-Aza-CdR treatment (methylation rate≤20％ );CHFR mRNA expression recovered after 5-Aza-CdR's demethylation.Conclusions CHFR gene's promoter display hypermethylation status in gastric cancer cell line AGS, 5-Aza-CdR can recovery abnormal hypermethylation and induce its mRNA expression significantly.It may be a new idea for the treatment of gastric cancer.关键词
5-氮杂-2'-脱氧胞苷/CHFR基因/去甲基化/胃癌/胃癌细胞Key words
5 -Aza-2'deoxycytidine/ CHFR gene/ demethylation/ gastric cancer/ gastric cancer cell分类
医药卫生引用本文复制引用
邱庆安,吕云福,陈一明,林友刚,伍海鹰,刘宁..5-氮杂-2'-脱氧胞苷对胃癌AGS细胞CHFR基因去甲基化作用及意义[J].山东医药,2011,51(14):6-7,2.
