安徽医科大学学报2011，Vol.46Issue(6)：549-552,4.
氨甲蝶呤对映体耐药A549细胞株中叶酰聚谷氨酸合成酶mRNA和蛋白表达
The mRNA and protein expression of folylpolyglutamate synthetase in methotrexate enantiomer-resistant A549 cell lines
摘要
Abstract
Objective To study the expression of folylpolyglutamate synthetase ( FPGS ) in methotrexate ( MTX ) enantiomer-resistant A549 cell lines [L-( + )-MTX and D-( - )-MTX ]. Methods The expression of FPGS on genetic and protein level was determined by FQ-PCR and Western blot in lung cancer A549 cells, and MTX enantiomer-resistant A549 cells [ L-( + )-MTX and D-( - )-MTX ], with the concentration of drug resistance was 15 μmol/L. Results The genetic expression level of FPGS was ( 0. 80 ±0. 09 ) and ( 2. 04 ±0. 34 ) folds in L-( + )MTX/A549 cells and D-( - )-MTX/A549 cells compared with lung cancer A549 cells, there was statistical difference between two groups ( P ＜ 0. 05 ). The protein expression level of FPGS was ( 0. 85 ±0. 12 ) and( 1. 62 ± 0. 24 ) folds in L-( + )-MTX/A549 cells and D-( - )-MTX/A549 cells compared with lung cancer A549 cells.there was statistical difference ( P ＜0. 05 ). Conclusion The expression level of FPGS on genetic and protein level in drug resistant cells have been changed, and significant difference in two enantiomer-resistant cells are appeared.关键词
肺肿瘤/氨甲蝶呤分类
医药卫生引用本文复制引用
孙利,沈佐君,何晓东,孙余婕,许维东,李道静,张白银,张永娟..氨甲蝶呤对映体耐药A549细胞株中叶酰聚谷氨酸合成酶mRNA和蛋白表达[J].安徽医科大学学报,2011,46(6):549-552,4.基金项目
国家自然科学基金(编号:30672011) （编号:30672011）
安徽省自然科学基金(编号:050430902) （编号:050430902）
安徽省人才开发资金(编号:2005Z040) （编号:2005Z040）
