中国肿瘤生物治疗杂志2011，Vol.18Issue(2)：144-148,5.DOI:10.3872/j.issn.1007-385X.2011.02.005
新城疫病毒7793株抑制人结肠癌LoVo细胞裸鼠移植瘤的生长及其机制
Inhibitory effect of Newcastle disease virus 7793 strain on human colon carcinoma LoVo cell-transplanted tumors in nude mice and the possible mechanism
摘要
Abstract
Objective: To investigate the effects of Newcastle disease virus 7793 strain ( NDV 7793) on the growth of human colon carcinoma LoVo cell-transplanted tumors in nude mice and the possible mechanism. Methods: Mouse models of LoVo cell-transplanted tumor were established and were randomly divided into 3 groups: intravenously injected with PBS, 5-FU and NDV 7793 groups. Tumor growth was observed in different groups, the apoptosis and necrosis rates of tumor cells were detected by FCM, expressions of Bax and Bcl-2 proteins were analyzed by immunohistochemical method,cyto-C level in tumor tissues was detected by cyto-C kit, and the concentration of TNF-α in tumor tissues was examined by ELISA. Results:NDV 7793 significantly inhibited the growth of LoVo-transplanted tumors compared with 5-FU (50.14％ vs 37. 14％, P ＜ 0.05 ). The apoptosis rate of LoVo-transplanted tumor cells in NDV 7793 group was significantly higher than that in 5-FU group ( [ 28.7 ± 1.5 ] ％ vs [ 1.46 ± 0.3 ] ％ ), and LoVo-transplanted tumor cells had a similar apoptosis rate and necrosis rate in NDV 7793 group ( [ 28.7 ± 1.5 ] ％ vs [ 27.80 ± 3.32 ] ％ ). NDV 7793 enhanced the expression of Bax, but not Bcl-2, in LoVo-transplanted tumor tissues, NDV 7793 also increased the cyto-C ( [ 2.28 ± 0. 68 ]vs [0.68 ±0. 13] μg/μl) and TNF-α levels ( [489.6±5.2] vs [ 167.9 ±3.9] pg/ml) in LoVo-transplanted tumor tissues. Conclusion: NDV 7793 can inhibit the growth of human colon carcinoma LoVo cell-transplanted tumors, which may be related to the up-regulation of Bax, cyto-C and TNF-α and the subsequent apoptosis of tumor cells.关键词
新城疫病毒/结肠癌/移植瘤/凋亡/坏死分类
医药卫生引用本文复制引用
肖庆,黄川,樊晓晖,宋德志,梁莹,宫金伶,王立芳,刘金颖,赖振屏..新城疫病毒7793株抑制人结肠癌LoVo细胞裸鼠移植瘤的生长及其机制[J].中国肿瘤生物治疗杂志,2011,18(2):144-148,5.基金项目
国家自然科学基金资助项目(No.30860328) （No.30860328）
广西教育厅科研项目(No.200810MS059). （No.200810MS059）
