中国全科医学2011，Vol.14Issue(18)：2037-2039,3.
结直肠癌患者门静脉血p27基因甲基化检测及其临床意义
Detection of p27 Gene Promoter Methylation in Portal Vein Blood and Its Significance in Diagnosis of Colorectal Carcinoma
摘要
Abstract
Objective To investigate the methylation status of p27 gene promoter in portal vein blood and peripheral blood of colorectal carcinoma patients and its relationship with the clinical characteristics of the disease.Methods The methylation status of p27 gene promoter in portal vein blood in 106 patients with colorectal carcinoma was detected using methylation — specific PCR ( MSP ).Blood samples from 29 age - matched healthy subjects were adopted as controls.Results Positive rate of p27 gene methylation in the peripheral blood of colorectal carcinoma patients were merely 24.5％ ( 26/106 ), compared to 3.4％ ( 1/29 ) in the control subjects, while in portal vein blood the positive rate went up to 30.2％ ( 32/106 ).Positive rates were 20.0％ ( 11/55 ) and 41.2％ ( 21/51 ) in patients of Duke A + B and Duke C + D, 48.0％ ( 12/25 ) and 24.7％ ( 20/81 ) in patients with low and middle -high differentiation, 41.5％ ( 17/41 ) and 23.1％ ( 15/65 ) in patients with and without lymph node metastasis, and 24.1％ ( 7/29 ) and 32.5％ ( 25/77 ) in patients with and without serosa invasion, respectively.Hyper-methylation status in p27 promoter was significantly correlated to staging, degree of differentiation, and status of lymph node metastasis ( P ＜0.05 ) and it was not significantly correlated to age, sex, neoplasm position and size ( P ＞0.05 ).Conclusion Hypermethylation in p27 gene promoter is associated with pathogenesis and development of colorectal carcinoma, which in turn might provide evidences for diagnosis and prognostic prediction of the disease.关键词
结直肠肿瘤/门静脉/p27基因分类
医药卫生引用本文复制引用
陈积贤,黄建武,梁美珍,贺先伟,徐平,张洁,任振华,薛迪新,吴伟力,张仁虎,余铭,林道浙,林肖..结直肠癌患者门静脉血p27基因甲基化检测及其临床意义[J].中国全科医学,2011,14(18):2037-2039,3.基金项目
温州科委课题基金(200703Y20070068) （200703Y20070068）
安徽省教育厅课题基金(2006 KJ077C) （2006 KJ077C）
