青岛大学医学院学报2011，Vol.47Issue(3)：210-212,215,4.
pIRES-Bax-HGF重组质粒的构建
CONSTRUCTION OF RECOMBINANT PLASMID PIRES-BAX-HGF
黄涛 1常青 1徐平1
作者信息
- 1. 青岛大学医学院附属医院心血管外科,山东,青岛,266003
- 折叠
摘要
Abstract
Objective To construct the plasmid vector of pIRES-Bax-HGF. Methods The Bax gene sequence was cloned from ovarian cancer samples, and HGF gene sequence from pBluescript Ⅱ SK+HGF plasmid derived from ATCC. Bax and HGF coding sequence were inserted into pIRES plasmid by step double enzyme digestion. Results Enzyme digestion and sequencing indicated that the construction of recombinant pIRES-Bax-HGF plasmid was successful. Conclusion Obtaining the coding genes of both Bax and HGF, and successfully creating the plasmid vector of plRES-Bax-HGF establish a foundation for further study of the effects of HGF and Bax on vascular endothelial cells, smooth muscle cells, and fibrocytes, which steps out an important step to treat post-CABG restenosis at gene level.关键词
卵巢肿瘤/肝细胞生长因子/bcl-2相关X蛋白质/质粒Key words
ovary neoplasms/ hepatocyte growth factor/ bcl-2-Associated X protein/ plasmids分类
医药卫生引用本文复制引用
黄涛,常青,徐平..pIRES-Bax-HGF重组质粒的构建[J].青岛大学医学院学报,2011,47(3):210-212,215,4.
