中国肿瘤生物治疗杂志2011，Vol.18Issue(2)：165-169,5.DOI:10.3872/j.issn.1007-385X.2011.02.009
骨唾液酸蛋白对乳腺癌MDA-MB-231细胞P13K-AKT信号通路的影响
Effect of bone sialoprotien on P13K-AKT signaling pathway of breast cancer MDA-MB-231 cells
摘要
Abstract
Objective: To investigate the effect of bone sialoprotein (BSP) on the PI3K-AKT signaling pathway in breast cancer MDA-MB-231 cells.Methods: BSP-silenced MDA-MB-231 cells were treated with recombinant human BSP (rhBSP) and the PI3K-AKT specific inhibitor LY294002.Western blotting analysis was used to detect the phosphorylation of AKT, qPCR was conducted to evaluate caspase-3, cyclin D1 mRNA expressions, and the proliferation of cells was analyzed by MTT assay.Results: Compared with the 231 BO-Scrambled cells in control group, BSP protein expression in BSP-silenced 231BO-BSP27 cells was significantly lower (74.32 ± 2.18 ) ％ ( P ＜ 0.01 ), and expression level of AKT phosphorylation was also significantly lower ( 33.30 ± 2.61 ) ％ ( P ＜ 0.01 ), resulting in up-regulation of caspase-3 mRNA level ( 1.000 ± 0.000 vs 1.733 ± 0.039, P ＜ 0.01 ) , down-regulation of cyclin D1 mRNA ( 1.000 ± 0.000 vs 0.370 ± 0.012, P ＜ 0.01 ), and the inhibition of 231BO-BSP27 cells growth ( P ＜ 0.05 ).After treatment with exogenous rhBSP, the phosphorylation of AKT was increased in both 231BO-Scrambled and 231BO-BSP27 cells [(17.86 ± 2.27) ％, (33.78 ± 1.51 ) ％, P ＜ 0.01].rhBSP treatment decreased caspase-3 mRNA ( 1.000 ± 0.039 vs 0.541 ± 0.091, P ＜0.01 ) , and increased cyclin D1 mRNA ( 1.000 ±0.000 vs 2.921 ±0.032, P ＜0.01 ) expression in 231BO-BSP27 cells, and stimulated the proliferation of 231 BO-Scrambled and 231BO-BSP27 cells ( P ＜ 0.01 ).Furthermore, rhBSP-induced activation of AKT was reversed by LY294002 in 231BO-Scrambled and 231BO-BSP27 cells ( P ＜ 0.05 ),with an increase in caspase-3 mRNA and decrease in cyclin D1 mRNA expression in 231BO-BSP27 cells ( all P ＜0.01 ),causing proliferation inhibition in 231 BO-Scrambled and 231 BO-BSP27 cells ( P ＜ 0.01 ).Conclusion: BSP can regulate the mRNA expressions of caspase-3 and cyclin D1, and affect the proliferation of breast cancer MDA-MB-231 cells through the PI3K-AKT signaling pathway.关键词
骨唾液酸蛋白/乳腺癌细胞/LY294002/PI3K-AKT信号通路分类
医药卫生引用本文复制引用
杨德猛,王捷,彭远远,王丽,夏冰,张宏斌,杨传红,张海燕..骨唾液酸蛋白对乳腺癌MDA-MB-231细胞P13K-AKT信号通路的影响[J].中国肿瘤生物治疗杂志,2011,18(2):165-169,5.基金项目
广东省自然科学基金项目(No.06104396). （No.06104396）
