中国肿瘤生物治疗杂志2011，Vol.18Issue(2)：170-174,5.DOI:10.3872/j.issn.1007-385X.2011.02.010
顺铂耐药促进卵巢癌OVCAR-3细胞的增殖、侵袭和迁移
Cisplatin-resistance increases proliferation,invasion and migration of ovarian cancer OVCAR-3 cells
摘要
Abstract
Objective: To investigate the differences in biological behavior between cisplatin-sensitive (OVCAR-3 cells) and cisplatin-resistant human ovarian cancer cells (OVCAR-3/CDDP cells), and to explore the possible mechanisms. Methods: OVCAR-3 and OVCAR-3/CDDP cells in logarithmic phase were collected, their proliferation were assessed by soft agar colony formation assay, their in vitro and in vivo invasion and migration abilities were measured by Transwell chamber assay, anoikis assay and subcutaneous transplanted-tumor formation assay in nude mice, and the expressions of MMP-2 and MMP-9 in transplanted tumor tissues were examined by immunohistochemical assay. Results:Colony formation rate of OVCAR-3/CDDP cells was significantly increased compared with OVCAR-3 cells ( [ 0.66％ ±0.09％ ] vs [0.31％ ±0.07％ ], P ＜ 0.05 ). Compared with OVCAR-3 cells, OVCAR-3/CDDP cells had significantly higher migration capability ( [233.1 ± 8.5 ]vs [ 167.4 ± 5.9 ], P ＜ 0.01 ) and invasive capability ( [ 143.6 ± 9.1 ] vs [95.8 ±6.2], P ＜0.01 ). OVCAR-3/CDDP cells tended to aggregate and their apoptosis index was decreased compared with that OVCAR-3 cells ( [ 7.78 ± 1.32 ] ％ vs [ 15.41 ± 1.26 ] ％, P ＜ 0.01 ). The expressions of MMP-2 and MMP-9 in transplanted tumor tissues of OVCAR-3/CDDP cell group were up-regulated compared with those of OVCAR-3 cell group (P ＜ 0.05). Conclusion: The proliferation, anti-anoikis, invasion and migration abilities of cisplatin-resistant OVCAR-3/CDDP cells are greatly increased, which might be related with the up-regulation of MMP-2 and MMP-9.关键词
卵巢癌/耐药/增殖/失巢凋亡/侵袭/迁移分类
医药卫生引用本文复制引用
王丹,惠宁..顺铂耐药促进卵巢癌OVCAR-3细胞的增殖、侵袭和迁移[J].中国肿瘤生物治疗杂志,2011,18(2):170-174,5.基金项目
全军医学科学技术研究"十一五"面上课题(No.MB2151). （No.MB2151）
