中国肿瘤生物治疗杂志2011，Vol.18Issue(2)：175-180,6.DOI:10.3872/j.issn.1007-385X.2011.02.011
bFGF单抗联合放疗对小鼠B16移植瘤的协同抑制作用
Synergistic inhibitory effects of bFGF monoclonal antibody combined with radiotherapy on B16-transplanted tumors in mice
摘要
Abstract
Objective: To investigate the inhibitory effect of basic fibroblast growth factor monoclonal antibody ( bFGFmAb) combined with radiotherapy against B16-transplanted tumors in mice. Methods: bFGF-mAb was prepared and purified. B16-transplanted melanoma tumor models were established and the mice were randomly divided into 4 groups: control group, radiotherapy group, bFGF-mAb group, and bFGF-mAb combined with radiotherapy group. Tumor volumes were measured in different treatment groups. Twenty days after treatment, the tumors were collected and weighted, and the inhibitory rates of tumor growth were calculated. TUNEL staining was used to detect the apoptosis rate of transplanted tumors; immunohistochemical method was used to examine the positive expression of bFGF, vascular endothelial growth factor (VEGF) and microvessel density (MVD) in transplanted tumor tissues. Results: The inhibitory rate of tumor growth in the combined treatment group was significantly higher than those in radiotherapy group and bFGF-mAb treatment group (48.76％ vs 12.17％, 30.49％, P ＜ 0.05 ). The radiotherapy sensitization enhancement ratio of the combined treatment group was 2.37 times that in the radiotherapy group. The apoptosis rate of transplanted tumor cells in the combined treatment group was significantly increased compared with those in the control, radiotherapy, bFGF-mAb treatment groups ([58.56±6.47]％ vs [17.21 ±2.86]％, [28.45±5.47]％, [10.62±1.73]％; P＜0. 05 orP＜0.01),with bFGF, VEGF expression and MVD being significantly decreased (P ＜ 0. 05 ). Conclusion: bFGF-mAb combined with radiotherapy have synergistic inhibitory effect on the growth of B16-transplanted melanoma tumors, and it can increase the radio-sensitivity of tumor cells by reducing the expressions of bFGF and VEGF, decreasing angiogenesis, and promoting apoptosis.关键词
黑素瘤/成纤维细胞因子单克隆抗体(bFGF-mAb)/放射治疗/血管内皮生长因子(VEGF)/肿瘤微血管密度(MVD)分类
医药卫生引用本文复制引用
曾世彬,徐萌,潘兰红,向军俭,邓宁,李丹,王盼盼..bFGF单抗联合放疗对小鼠B16移植瘤的协同抑制作用[J].中国肿瘤生物治疗杂志,2011,18(2):175-180,6.基金项目
国家高技术研究发展计划(863计划)资助项目(No.2009AA022112). （863计划）
