中国农业科学2011，Vol.44Issue(6)：1085-1091,7.DOI:10.3864/j.issn.0578-1752.2011.06.002
基于GISH的甘蔗与蔗茅属间杂交F1后代染色体组成及核型分析
Chromosomal Constitution and Karyotype of Intergeneric F1 Hybrids Between Saccharum spp.and Erianthus fulvus Based on GISH
摘要
Abstract
[Objective] The objective of current experiment was to study the chromosomal constitution and karyotype of F1 hybrids that derived from sexual cross between cultivated sugarcane (Saccharum spp.) and Erianthusfulvus. [Method] Both monocolor and bicolor GISH (Genomic in situ hybridization) were performed using digoxigenin-labeled E. fulvus DNA and biotin-labeled female parental DNA as probes, the karyotypes of 3 hybrids were analyzed based on the result of GISHs. [ Result ] The results of GISHs revealed the transmission of 7 to 10 E. fulvus chromosomes into progenies. The chromosomes of E. fulvus were broken because of the female parents prevented alien genetic materials from introgressing during distant hybridization and resulted in elimination and fragmentation of E. fulvus chromosomes. The GISH result on nuclear of progeny showed the chromatins came from male and female parent are not intermixed and stays at different regions. The karyotype formulas of 3 hybrids were 2n=92=80m (4SAT)+12sm.2n=88=82m+6sm and 2n=88=78m(2SAT)+10sm, and their karyotypes were 2C, 2C and 2B, respectively. [Conclusion]Chromosomal constitution of F1 hybrids that derived from sexual cross between cultivated sugarcane (Saccharum spp.) and E. fulvus were n+n and 2n+n. The results of karpotype analysis showed that the chromosomes of these three progenies are primitive.关键词
甘蔗/蔗茅/F1子代/基因组原位杂交/核型Key words
Saccharum spp./ Erianthus fulvus/ F1 hybrids/ GISH/ karyotype引用本文复制引用
王先宏,杨清辉,李富生,何丽莲,何顺长..基于GISH的甘蔗与蔗茅属间杂交F1后代染色体组成及核型分析[J].中国农业科学,2011,44(6):1085-1091,7.基金项目
国家自然科学基金项目(30960045)、云南省人才培养计划项目(2006PY-38)、云南省现代农业甘蔗产业技术体系建设项目(2009-2013) （30960045）
