军医进修学院学报2011，Vol.32Issue(5)：496-498,500,4.DOI:CNKI:11-3275/R.20110224.1023.000
携带SPK1基因的重组腺病毒对犬酒精性胃溃疡愈合的影响
Effect of sphingosine kinase-1 gene recombinant adenovirus on healing of gastric ulcer induced by alcohol in experimental dogs
摘要
Abstract
Objective To study the therapeutic effect of Ad-sphingosine kinase-l(SPKl) gene on alcohol-induced gastric ulcers. Methods A dog model of alcohol-induced gastric ulcer was established. Adenovirus carrying green fluorescent protein(GFP) was prepared and used as a control to observe the distribution of GFP in gastric wall of dogs. Ulcer healing was observed under an electronic gastroscope 7,14, 21 and 28d respectively, after the drugs were given Ad-GFP or Ad-SPKl. SPK1 protein expression in gastric tissue was detected by Western blot. Activity of cellular SPK.1 was assayed with γ -P32 ATP incorporation method. Expression of CD34 was detected with immunohistochemical method and microvessel density(MVD) was calculated. Results Adenovirus could effectively mediate the expression of GFP and SPK1 in gastric mucosa cells. Adenovirus-mediated Ad-SPKl gene transfer promoted the healing of gastric ulcer under electronic gastroscope. The MVD was significantly higher in Ad-SPKl group than in control group(P<0.05). Conclusion Ad-SPKl can promote gastric mucosa repair and new vascular formation, thus accelerating the healing of ulcers in experimental dogs.关键词
胃溃疡/胃镜/鞘氨醇激酶/基因治疗/愈合Key words
Stomach Ulcer/ Gastroscopes/ Sphingosine Kinase-1/ Gene Therapy/ Healing分类
临床医学引用本文复制引用
郭强,张凯,孔庆龙,唐平,张修礼,孙刚..携带SPK1基因的重组腺病毒对犬酒精性胃溃疡愈合的影响[J].军医进修学院学报,2011,32(5):496-498,500,4.基金项目
国家自然科学基金基金项目(30901462) （30901462）
