中国体外循环杂志2011，Vol.9Issue(2)：89-92,4.
γ-分泌酶抑制剂对正常乳鼠心肌细胞的影响
The effects of DAPT treatment on cardiomyocytes from neonatal rats
摘要
Abstract
OBJECTIVE To investigate the effects of N - ( N - ( 3 .5 - difluorophenacetyl ) - 1 - alanyl ) - s - phenylglycine t - butyl ester ( DAPT ) treatment on normal cardiomyocytes from neonatal rats. METHODS Cardiomyocytes from neonatal rats were separated and cultured in vitro. The cells were incubated with different concentrations ( in μmol/L : 10 , 5 , 2. 5 , 1. 25 , 0. 63 , 0. 31 ,0. 16 ) of DAPT for 24 h. or incubated with DAPT ( 5 μmol/L ) for 1 h, 2 h, 4 h, 8 h. 16 h, 24 h and 48 h. The viability of the cells was investigated with MTT method. The expression of Notch intracellular domain ( NICD ) and Bcl - 2 protein were detected with Western - Blotting. RESULTS The viability of neonatal rat cardiomyocytes was significantly depressed with DAPT incubation. The effects were positively related to the concentrations of DAPT and the time of incubation. DAPT treatment also significantly decreased the expression of NICD and Bcl - 2 protein. CONCLUSION DAPT can interrupt the transduction of Notch signaling pathway in neonatal rat cardiomvocytes and inhihit the viability of the cells and increase their apoptosis.关键词
乳鼠/心肌细胞/DAPT/NICD/Bcl-2Key words
Neonatal rat/ Cardiomyocytes/ DAPT/ NICD/ Bcl - 2分类
医药卫生引用本文复制引用
杨阳,段维勋,周京军,毕生辉,杨建宝,冯建宇,侯晓薇,金振晓,易定华..γ-分泌酶抑制剂对正常乳鼠心肌细胞的影响[J].中国体外循环杂志,2011,9(2):89-92,4.基金项目
陕西省自然科学基金(2007C248) （2007C248）
西京医院学科助推计划(XJZT09M16) （XJZT09M16）
