西安交通大学学报（医学版）2011，Vol.32Issue(3)：272-274,278,4.
T-2毒素中毒对低硒大鼠关节软骨细胞凋亡相关基因mRNA表达的影响
Effects of T-2 toxin on the mRNA expression of apoptosis-related gene in articular chondrocytes of selenium-deficiency rats
摘要
Abstract
Objective To study the effects of T-2 toxin on the mRNA expression of apoptosis-related gene in articular chondrocytes of selenium-deficiency rats for providing some evidence for understanding the mechanisms leading to chondrocyte apoptosis and the cause of Kashin-Beck disease. Methods Weaning male SD rats were randomly divided into four groups, i.e., normal diet group (Group A), selenium-deficient diet group (Group B),normal diet plus T-2 toxin group (Group C), and selenium-deficient diet plus T-2 toxin group (Group D). And then the rats in Groups A and C were fed with normal diet and those in Groups B and D were fed with seleniumdeficiency diet for 30 d. After that, rats in Groups C and D received an intragastric administration of T-2 toxin (200 ng/(g · d · bw) for 30 d, respectively. The articular cartilage was removed from rats and RNA was extracted from the articular cartilage by Trizol. Real time-PCR was used to determine the mRNA expressions of Bax, Bcl-2 and P53 and caspase-3. Results Compared with that of normal diet rats (73.92 ± 30.01)ng/mL, the blood selenium content was ( 4.16 ± 3.56 ) ng/mL in rats on selenium-deficient diet for 30 d, with a significant difference (P＜0.05). Compared to that in normal diet group, the mRNA expressions of P53, caspase-3, Bax and Bcl-2 were up-regulated in selenium-deficient diet group, normal diet plus T-2 toxin group and selenium-deficient diet plus T-2 toxin group. However, the mRNA expression of Bcl-2 was down-regulated, with a significant difference (P＜0.05). Compared to that in selenium-deficient diet group, there were up-regulated mRNA expressions of P53,caspase-3 and Bax in selenium-deficient diet plus T-2 toxin group, but down-regulated mRNA expression of Bcl-2;the differences showed significance (P ＜ 0.05). In selenium-deficient diet plus T-2 toxin group, the mRNA expressions of P53, caspase-3 and Bax were higher than those in normal diet plus T-2 toxin group, but the mRNA expression of Bcl-2 was significantly lower than that in normal diet plus T-2 toxin group (P＜0.05). Conclusion T-2 toxin (200 ng/(g · bw · d) can cause the mRNA expression change of chondrocytes apoptotic factors in rats on selenium-deficient diet.关键词
T-2毒素/硒缺乏/软骨细胞/细胞凋亡/P53/caspase-3/Bax/Bcl-2/大鼠分类
医药卫生引用本文复制引用
杨浩杰,马天有,王治伦,陈静宏,宋代琴,薛森海,周晓荣,陈群,谭希旺,杨占田..T-2毒素中毒对低硒大鼠关节软骨细胞凋亡相关基因mRNA表达的影响[J].西安交通大学学报（医学版）,2011,32(3):272-274,278,4.基金项目
国家自然科学基金重点项目资助(No.30630058) （No.30630058）
