中国体外循环杂志2011，Vol.9Issue(2)：102-104,128,4.
体外循环对大鼠血脑屏障及紧密连接蛋白表达的影响
Effects of cardiopulmonary bypass on blood-brain barrier and the expression of tight junction protein in rats
曹惠鹃 1孙莹杰 1张铁铮 1周锦 1姚婧1
作者信息
- 1. 110016,沈阳,沈阳军区总医院麻醉科
- 折叠
摘要
Abstract
OBJECTIVE To investigate the effects of cardiopulmonary bypass ( CPB ) on blood - brain barrier ( BBB ) and the expression of tight junction ( TJ ) protein of cerebral vascular endothelium in rat model. METHODS 24 adult male Sprague -Dawley rats were randomly divided into two groups: group S served as shame - operated group without CPB; the rats of group C underwent 60 minutes of CPB and were observed 2 hours later. The Evans blue content of brain tissue was measured in 6 rats of each group,the water content of brain tissue, the Occludin and ZO - 1 expression of brain tissue, and the ultrastructural of BBB were observed in the another 6 ones of each group. RESULTS Compared with group S, the Evans blue content and water content of group C significantly increased ( P ＜0. 05 ),the expression of Occludin and ZO - 1 of group C significantly decreased ( P ＜0. 05 ). The basement membrane of BBB of group S was uniform, but that of group C was swollen. The TJ between endothelia of group S was intact, but that of group C was unclear. CONCLUSION CPB destroys the structure of BBB and increases the permeahility of it, which may have relationship with the change of TJ protein.关键词
体外循环/紧密连接蛋白/血脑屏障Key words
Cardiopulmonary bypass/ Tight junction/ Blood - brain barrier分类
医药卫生引用本文复制引用
曹惠鹃,孙莹杰,张铁铮,周锦,姚婧..体外循环对大鼠血脑屏障及紧密连接蛋白表达的影响[J].中国体外循环杂志,2011,9(2):102-104,128,4.
