西安交通大学学报（医学版）2011，Vol.32Issue(3)：315-318,327,5.
三叶因子3在胃溃疡大鼠脾脏不同部位的表达
Expression of TFF3 in different positions of the spleen during gastric ulcer in rats
摘要
Abstract
Objective To study the expression of trefoil factor 3 (TFF3) in different positions of the spleen during gastric ulcer in rats.Methods Acetic acid-induced ulcer was induced by injecting acetic acid into submucosa of gastric anterior wall.The expression of TFF3 in the spleen of 6 normal rats and 42 rats with gastric ulcer was detected by immunohistochemistry.Results ① In gastric ulcer rats, the spleen was swollen and congested, and the area of white pulp (WP) was enlarged obviously (P＜0.05 or P＜0.01), which was the largest in the 6th-day group; the marginal zone (MZ) was thickened (P＜0.05 or P＜0.01), which was the thickest in the 10th-day group, but the area of red pulp (RP) was reduced.② The positive products of TFF3 were brown and yellow granules distributed in the cytoplasm.③ The expression of TFF3 in the spleen was weakly positive in normal group,but was obviously increased in gastric ulcer group (P＜0.05 or P＜0.01).The TFF3 average optical density (AOD)values in WP were obviously higher in each gastric ulcer group after gastric ulcer than in normal group (P＜0.05),which was the highest in the 10th-day group.The TFF3 AOD values in MZ were obviously higher in the 1st-day,2nd-day and 6th-day groups than in normal group (P＜0.05 or P＜0.01), which was the highest in the 6th-day group.The TFF3 AOD values in RP were obviously higher in the 1st-day, 2nd-day, 6th-day and 14th-day groups than in normal group (P＜0.05 or P＜0.01), which was the highest in the 6th-day group.④ The expression of TFF3 in MZ of gastric ulcer group had a negative correlation with injury time (P＜0.05).Conclusion TFF3 is highly expressed in different regions of the spleen during gastric ulcer in rats.Healing of gastric ulcer may be promoted by enhancing spleen immunoreactivity.关键词
脾脏/胃溃疡/三叶因子3/免疫组织化学/大鼠分类
医药卫生引用本文复制引用
张江兰,吴靖芳,王志勇,张静,吕洋,郑慧娥,张耕..三叶因子3在胃溃疡大鼠脾脏不同部位的表达[J].西安交通大学学报（医学版）,2011,32(3):315-318,327,5.基金项目
河北省自然科学基金资助项目(No.C200700906) （No.C200700906）
