西安交通大学学报（医学版）2011，Vol.32Issue(3)：340-343,347,5.
人颈椎间盘退变基因表达谱的研究
Gene expression pattern of cervical disc degeneration of humans
摘要
Abstract
Objective To investigate the gene expression changes in cervical intervertebral disc of humans and analyze the mechanism of disc degeneration. Methods The total RNAs were isolated by improved one-step method from human cervical disc of both 6 cases of degeneration and 6 normal ones. The transcribed cDNAs were labeled with the incorporations of fluorescent dNTP (Cy3, Cy5) for the probes. The cDNA microarray was scanned for the fluorescent signals by laser scanner and analyzed by computer image analysis after the mixed probes were hybridized to the microarray. Results There were 570 differentially expressed genes between degenerated disc and normal disc after 6 times of scanning. There were 550 up-regulated genes and 20 down-regulated genes.Conclusion At the gene level, abnormal expression of many genes is associated with cervical disc degeneration.关键词
颈椎间盘/退变/基因芯片/基因表达谱/差异表达分类
医药卫生引用本文复制引用
张小卫,郭团茂,刘淼,张银刚..人颈椎间盘退变基因表达谱的研究[J].西安交通大学学报（医学版）,2011,32(3):340-343,347,5.基金项目
陕西省医学会基金资助(No.2686) （No.2686）
