中国肿瘤生物治疗杂志2011，Vol.18Issue(2)：201-205,5.DOI:10.3872/j.issn.1007-385X.2011.02.016
胃癌组织中Toll样受体4/9的表达与肿瘤病理分期和转移的相关性
Correlation of Toll-like receptor 4/9 expression with pathological stages and metastases of gastric cancer
摘要
Abstract
Objective: To investigate the correlation of Toll-like receptors (TLR)4 and TLR9 expression with the pathological stages and metastases of gastric cancer. Methods: Forty-two gastric cancer and paracancerous tissues were included in the present study (patients were diagnosed in No. 174 Hospital of PLA from Jan. 1 2009 to Oct. 2009). The mRNA and protein expressions of TLR4 and TLR9 in the gastric cancer and paracancerous tissues were detected by RT-PCR and Western blotting analysis, respectively. Results: The expressions of TLR4 and TLR9 mRNA were significantly higher in the gastric cancer tissues than those in the paracancerous tissues ( 1.29 ± 0.03 vs 0. 53 ± 0.02, 0.99 ± 0.04 vs 0. 22 ± 0. 05 ;P ＜0.05). T3 ± T4 gastric cancer tissues demonstrated significantly higher levels of TLR4 and TLR9 mRNA than the T1 ± T2 tissues ( P ＜ 0.05 ). Significantly higher levels of TLR4 and TLR9 mRNA were observed in patients with distant metastases than in those without ( P ＜ 0.05 ). However, no correlation was observed between TLR4 and TLR9 mRNA expression with the age, gender or lymphatic metastasis status of patients. The expressions of TLR4 and TLR9 protein were significantly higher in the gastric cancer tissues than those in the paracancerous tissues ( 1.36 ± 0.05 vs 0. 48 ± 0.04,1.12 ±0.05 vs 0. 34 ±0.03 ;P ＜0.05 ), T3 ± T4 gastric cancer tissues demonstrated significantly higher levels of TLR4 and TLR9 proteins than the T1 ± T2 tissues (P ＜ 0.05 ). Conclusions: TLR4 and TLR9 are up-regulated in gastric carcinoma tissues, and TLR4/9 expression is correlated with the TNM staging and distant metastasis. These data suggest that TLR4/9 may be involved in the development and progression of gastric cancer, and TLR4/9 may be potential target for gastric cancer treatment.关键词
胃癌/Toll样受体4/Toll样受体9分类
医药卫生引用本文复制引用
陈战,涂永久,郭明,戚沛霖,戴益琛..胃癌组织中Toll样受体4/9的表达与肿瘤病理分期和转移的相关性[J].中国肿瘤生物治疗杂志,2011,18(2):201-205,5.基金项目
厦门市科技基金项目(No.KJ2010014). （No.KJ2010014）
