中国实验动物学报2011，Vol.19Issue(2)：95-99,后插2,6.DOI:10.3969/j.issn.1005-4847.2011.02.002
Exo-1基因对端粒酶缺陷小鼠造血干细胞植入效率的影响
Influence of Exo-1 gene on the chimerism rate in telomerase-defecient mice
摘要
Abstract
Objective To investigate the influence of Exo-1 gene deletion on the engraftment of hematopoietic stem cell (HSC) transplantation by different methods into the telomerase knockout mice. Methods Bone marrow cells or KSL ((c-kit+ ,Sca-1 + ,lineage) cells were isolated from CD45. 1 mice, and then injected int recipients, which were telomerase gene knockout or Exo-1 and telomerase gene double knockout mice with or without irradiation. At 1 month later, peripheral blood was collected and analyzed by flow cytometry. Results Low chimerisms of donor-derived cells were observed in recipients without irradiation or with 1 Gy, 2 Gy irradiation. With 6 Gy irradiation, the chimerisms were below 50％ , and died in 1 month. With 3 Gy irradiation, most mice lived with high chimerisms. Conclusions 3 Gy X-ray irradiation gives rise to high chimerisms in telomerase knockout mice, whereas Exo-1 gene does not further increase the chimer isms of HSC transplantation.关键词
端粒酶基因缺陷/骨髓移植/造血干细胞/衰老分类
生物科学引用本文复制引用
石桂英,陈显达,陈陟阳,鞠振宇..Exo-1基因对端粒酶缺陷小鼠造血干细胞植入效率的影响[J].中国实验动物学报,2011,19(2):95-99,后插2,6.基金项目
国家自然基金面上项目[30771189] （）
北京市科委科技新星计划[2008A115]. （）
