中国实验动物学报2011，Vol.19Issue(2)：100-102,后插3,4.DOI:10.3969/j.issn.1005-4847.2011.02.003
EERα基因敲除小鼠的繁育及子代小鼠基因型的鉴定
Breeding of ERα gene knockout mice and genotype identification of their filial generation
摘要
Abstract
Objective To breed and identify estrogen receptor α (ERα) knockout mice and to establish an animal experimental model to further study the important role of ERα. Methods ERα knockout mice were paired in different ways. Genomic DNA was isolated from tails and analyzed by PCR. Results The ratio of the offspring genotypes fits the Mendel's laws. The male and female ERα knockout homozygote (ERα-/- ) mice are infertile. The testes, uteri and ovaries were significantly different between ERα -/- and wild-type mice. The testis and uterus weights of ERα-/- mice were significantly lower than that of wild-type mice. Some seminiferous tubules had a dilated lumen and a thin lining layer of disorganized seminiferous epithelium with few spermatogenic cells. Histological examination showed the presence of stromal,epithelial, and myometrial tissues but in reduced size. The ovaries showed hemorrhagic follicles, but no corpora lutes were observed. Conclusions It is feasible to breed ERα knockout homozygote mice by inbreeding of the heterozygotes. PCR methods can be used to identify the genotype of the ERα -/- mice precisely.关键词
雌激素受体α/基因敲除小鼠/基因型分类
生物科学引用本文复制引用
王强,钱文溢,刘景丽,朱勤,程洁,王宇,丁海霞,肖杭..EERα基因敲除小鼠的繁育及子代小鼠基因型的鉴定[J].中国实验动物学报,2011,19(2):100-102,后插3,4.基金项目
国家自然科学基金项目(30771831,81072329) （30771831,81072329）
江苏省高校自然科学研究项目(09KJB310005) （09KJB310005）
江苏省普通高校研究生科研创新计划项目. （）
