中国实验动物学报2011，Vol.19Issue(2)：103-106,后插4,5.DOI:10.3969/j.issn.1005-4847.2011.02.004
小鼠心肌纤维化模型的制备及其胶原沉积的机制
Establishment of a mouse model of myocardial fibrosis with isoproterenol and the mechanism of collagen deposition
摘要
Abstract
Objective To establish a manipulation-easy animal model of post-isehemie myocardial fibrosis and investigate the mechanism of collagen deposition. Methods Twenty BALB/c mice were randomly divided into two groups:the experimental group and control group. The mice in the experimental group were subjected to hypodermic injection with isoproterenol in a dose of 50 mg/kg. The injection was carried out twice a day for consecutive 10 days. Physiological saline was injected with the same method in the control group. The surface electrocardiogram was measured. Forty-five days after injection, collagen distribution was observed using sirius red staining. Expression levels of matrix metalloproteinase-9 (MMP-9) And tissue inhibitor of metalloproteinase-1 (TIMP-1) in myocardium were detected by fluorescent qRT-PCR.The expression of laminin (LN) in the heart, liver and kidney was detected by immunohistochemistry. Results The experimental group had increased ventricular arrhythmia and faster heart rate, and had increased levels of MMP-9, TIMP-1, LN and collagen deposition in the myocardium than those in the control group(P ＜0. 05). The expression of LN in the liver and kidney showed no significant difference ( P ＞ 0. 05 ). Conclusions Isoproterenol-induced collagen deposition in the experiment is a reproducible, handy and credible method to establish an animal model of myocardial fibrosis. MMP-TIMP imbalance is involved in the mechanism.关键词
异丙肾上腺素/心肌纤维化/小鼠/动物模型分类
生物科学引用本文复制引用
邓玮,陈庆伟,李兴升,李桂琼,柯大智,莫显刚..小鼠心肌纤维化模型的制备及其胶原沉积的机制[J].中国实验动物学报,2011,19(2):103-106,后插4,5.基金项目
国家自然科学基金项目(编号:30970826). （编号:30970826）
