中国康复理论与实践2011，Vol.17Issue(3)：219-222,4.
甲基强的松龙对大鼠脊髓损伤后星形胶质细胞的影响
Effects of Methylprednisolone on Astrocyte after Spinal Cord Injury in Rats
摘要
Abstract
Objective To investigate the effects of methylprednisolone (MP) on the proliferation and expression of glial fibrillary acidic protein (GFAP) in astrocytes after spinal cord injury in rats. Methods The spinal cords in T8 ～T10 in 48 male Wistar rats (10 weeks old) were exposed and the right site were semi-transected. 24 rats were administrated MP through tail vein as experiment group, and the other rats were injected equal volumes of normal saline as the control group. They were assessed with Basso, Beattie,and Bresnahan (BBB) score for ethological test every 4 d, and were sacrificed, perfused 3 d, 7 d, 14 d and 28 d after operation. The spinal cords were removed and postfixed, The expression of GFAP was observed with immunohistochemical staining and gray values determination. The GFAP positive cells were also counted. Results There was no significant difference in BBB score between these groups until 21 d after operation; but the MP group rats showed significantly better functional recovery than the control group 25 d after operation. The number of positive astrocytes was less in the MP group than in the control group 3 and 7 d after operation, but there was no significant difference 14 d after operation. The GFAP expression in glial scar was significantly lower in the MP group than in the control in the first 21 d after operation, but no significant difference 28 d after operation. Conclusion The large dose methylprednisolone administration can decrease the number of active AS, attenuate the formation of gliai scars, and improve the functional recovery of the hind limbs after acute spinal cord injury.关键词
脊髓损伤/甲基强的松龙/胶质纤维酸性蛋白/星形胶质细胞/大鼠分类
医药卫生引用本文复制引用
白旭东,李洪鹏,高杰,巴方,刘宁,孙永新..甲基强的松龙对大鼠脊髓损伤后星形胶质细胞的影响[J].中国康复理论与实践,2011,17(3):219-222,4.基金项目
1.国家自然科学基金资助项目(30872669) （30872669）
2.辽宁省教育厅资助项目(2008778). （2008778）
