中国中西医结合杂志2011，Vol.31Issue(4)：537-541,5.
Wnt/β-catenin信号通路相关蛋白在补肾活血颗粒含药血清影响成骨细胞过程中的表达及意义
The Expression and Significance of Wnt/β-catenin Signal Pathway Protein in the Effect of Bushen Huoxue Granule Containing Serum on the Osteoblast
摘要
Abstract
Objective To investigate the effect of Wnt/β-catenin signal pathway protein in the effect of Bushen Huoxue Granule (BHG) containing serum on the osteoblast, and to provide necessary experimental reliance for its action of mechanism in treatment of osteoporosis. Methods The osteoblast from cranial bones of neonates rat were isolated and cultured in vitro, which was divided into the blank control group and the BHG containing serum group. The alkaline phosphatase (ALP) activities of the osteoblast in each group were quantitatively detected and the ALP staining was performed six days later. The alizarin red staining was performed eighteen days later. At the same time, levels of Wnt/β-catenin signal pathway protein-β-catenin, low density lipoprotein correlated protein 5 ( LRP 5), and T cell factor (TCF) of osteoblasts in each group were detected by ELISA.Results BHG containing serum could significantly increase the expression of ALP and promote the formation of mineralizing nodus in the osteoblasts. At the same time it also markedly up-regulated the expressions of β-catenin, LRP 5, and TCF in this process. Conclusion BHG containing serum could markedly increase ossify activities and mineralization of osteoblast. This action was closely correlated with Wnt/β-catenin signal pathway. So it indicated that Wnt/β-catenin signal pathway played a very important role in the treatment of the osteoporosis by BHG.关键词
Wnt/β-catenin信号通路/补肾活血颗粒/成骨细胞Key words
Wnt/β-catenin signal pathway/ Bushen Huoxue Granule/ osteoblast引用本文复制引用
许应星,吴承亮,吴岩,童培建,肖鲁伟..Wnt/β-catenin信号通路相关蛋白在补肾活血颗粒含药血清影响成骨细胞过程中的表达及意义[J].中国中西医结合杂志,2011,31(4):537-541,5.基金项目
浙江省中医药科技计划重大项目(No.2006Z003) （No.2006Z003）
浙江省中医药现代化专项资金(浙经信医化[2010]421号) （浙经信医化[2010]421号）
