广东牙病防治2011，Vol.19Issue(4)：192-196,5.
两种正畸力作用下大鼠牙周组织中胶原相关蛋白的表达
Effect of Different Orthodontic Force on the Expression of Collagen Related Proteins in Periodontium of Rat
刘楚峰 1曹阳 2王军 3赵志河3
作者信息
- 1. 广东省口腔医院·南方医科大学附属口腔医院正畸科,广东,广州,510280
- 2. 中山大学光华口腔医学院·附属口腔医院正畸科
- 3. 四川大学华西口腔医学院
- 折叠
摘要
Abstract
Objective To explore the rebuilding mechanism during the orthodontic tooth movement by investigating the variation of the expression of collagen Ⅰ , MMP-1 and TIMP-1 in rat periodontal tissues. Methods Orthodontic appliance was placed between the left maxillary first molar and maxillary central incisors of rats. Groups were made according to orthodontic force and tooth movement period of 3 h, 6 h, 12 h, 1 d, 3 d, 7 d and 14 d. Collagen Ⅰ and MMP-1、TIMP-1 expression were examined with immunohistochemical technique and image analysis system. Results Under the application of mechanical force, the collagen Ⅰ and MMP-1, TIMP-1 expression in the periodontal tissues were more stringer stained than those of the controls, and manifested time-dependence. Multiple regression analysis results displayed MMP-1 and MMP-1/TIMP-1 showed negative correlative direction with collagen Ⅰ , and TIMP-1 showed positive correlative direction with collagen Ⅰ in the tension zones. Conclusion The results suggested that MMP-1 and TIMP-1 regulated the expression of collagen Ⅰ in the periodontal remodeling during tooth movement. The level of MMP-1/TIMP-1 played an essential role in maintaining the periodontal health and normal rebuilding.关键词
正畸牙移动/Ⅰ型胶原/基质金属蛋白酶-1/金属蛋白酶组织抑制因子-1/免疫组织化学Key words
Experimental tooth movement/ Collagen Ⅰ/ Matrix metalloproteinase-1/ Tissue inhibitor of metalloproteinase-1/ Immunohistochemistry分类
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刘楚峰,曹阳,王军,赵志河..两种正畸力作用下大鼠牙周组织中胶原相关蛋白的表达[J].广东牙病防治,2011,19(4):192-196,5.
