中国药房2011，Vol.22Issue(17)：1562-1563,2.
HPLC法测定大鼠肝微粒体中洛伐他汀含量的方法学研究
Methodological Study of Content Determination of Lovastatin in Rat Liver Microsomes by HPLC
姚卓贤 1夏宗玲 2于锋2
作者信息
- 1. 中国药科大学,南京市210009
- 2. 江苏常州市第一人民医院药剂科,常州市213001
- 折叠
摘要
Abstract
OBJECTIVE: To investigate the methology of the content determination of lovastatin in rats liver microsomes.METHODS: HPLC method was adopted with diazepam as internal standard.The determination was performed on UltimateXB-C18 column with mobile phase consisted of acetonitrile-water (70:30) at a flow rate of 1.0 mL· min-1.The detection wavelength was set at 238 nm and column temperature was 30 ℃.The injection volume was 20 μL.RESULTS: The calibration curves of lovastatin were linear in the range of 2.5～40 μmol· L-1 (r=0.998 3).The intra-day RSD of lovastatin at low, medium and high concentrations were 5.44％, 2.89％ and 2.83％, and the inter-day RSD were 5.48％, 1.84％ and 1.91％, respectively.The relative recoveries were ( 109.24 ± 3.28) ％, ( 104.82 ± 5.92 ) ％, ( 102.17 ± 1.92 ) ％, and the absolute recoveries were ( 104.65 ± 2.93) ％, ( 113.72 ± 6.25) ％, (101.92 ± 1.89)％, respectively.CONCLUSION: Results of methological study show that the method is simple, sensitive and suitable for the content determination of lovastatin in rat liver microsomes.关键词
高效液相色谱法/洛伐他汀/大鼠/肝微粒体/含量测定分类
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姚卓贤,夏宗玲,于锋..HPLC法测定大鼠肝微粒体中洛伐他汀含量的方法学研究[J].中国药房,2011,22(17):1562-1563,2.
