中国实验动物学报2011，Vol.19Issue(2)：111-114,后插5,5.DOI:10.3969/j.issn.1005-4847.2011.02.006
2型糖尿病大鼠的尿液代谢组学改变
Urinary metabonomic variation in type 2 diabetic rats
摘要
Abstract
Objective To investigate the urinary metabonomic variation in diabetic rats in order to explore the application of metabonomics in research of diabetes. Methods Male Sprague-Dawley rats were fed with high-fat and high-sucrose diet for 6 weeks and then injected with vehicle or streptozotocin (STZ) in a dose of 37 mg/kg. Fasting blood glucose (FBG) was monitored weekly after STZ injection. Urines of normal rats and model rats were collected at 1, 2, 4, 6, 8 weeks after STZ injection. Serum insulin (INS), triglyceride (TG), total cholesterol (TC) and free fatty acids (FFA) were measured at 8 weeks after STZ injection and the rats were sacrificed. The metabolic profiles were analyzed using ethyl chloroformate (ECF)derivatization and gas chromatography-mass spectrometry (GC/MS). Results Compared with the control rats, the FBG, TG, FFA were significantly higher while the INS was reduced in the model rats. The metabolism profiles of the model rats were significantly different from that of the control rats, and changed with time. Comparative study was applied and ten biological markers were different between the normal and model rats. Conclusions Significant changes in urine metabonomics occurre in type 2 diabetic rats induced by high-fat and high-sucrose diet and low dose of STZ,and it may reflect to a certain extent the pathological changes of type 2 diabetic rats.关键词
2型糖尿病/大鼠/高糖高脂喂养/链脲菌素/代谢组学分类
生物科学引用本文复制引用
袁琳,白永生,周明眉,胡娜,陆雄..2型糖尿病大鼠的尿液代谢组学改变[J].中国实验动物学报,2011,19(2):111-114,后插5,5.基金项目
上海市重点学科建设项目(编号:T0301) （编号:T0301）
上海高校选拔培养优秀青年教师科研专项基金(编号:szy08043). （编号:szy08043）
