中国药房2011，Vol.22Issue(17)：1569-1571,3.
固相萃取-HPLC法测定MCF-7细胞内、外液中多西他赛的含量
Content Determination of Docetaxel in MCF-7 Cell Extracellular and Intracellular Fluid by SPE-HPLC
柯昌毅 1车坷科 1王丽娟2
作者信息
- 1. 重庆市第三人民医院,重庆市400014
- 2. 重庆医药高等专科学校,重庆市400030
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for the content determination of docetaxel (DOC) in extracellular and intracellular fluid of human breast cancer cell MCF-7.METHODS: HPLC method was adopted.The samples of extracellular and intracellular fluid were prepared and the protein was removed using trichioroacetic acid.The DOC was extracted by solid phase extraction column (SPE).The samples were separated on a symmetry-C18 column with mobile phase consisted of potassium phosphate buffer-acetonitrile (50:50, V/V).The column temperature was 30 ℃ and detection wavelength was set at 230 nm.RESULTS: The linear range of DOC in extracellular and intracellular fluid was 0.05～5.50 μg· mL-1 (r=0.999 3, r=0.999 0) with average recovery more than 94％ (n=5).The RSD of intra-day and inter-day were lower than 5％ (n=5).The lowest detection limits were 0.05 μg· mL-1 and 0.03 μg·mL-1.The content of DOC in intracellular fluid was much higher than in extracellular fluid.CONCLUSION: The method is simple, sensitive and with little interference, and could be applied for the content determination of DOC in extracellular and intracellular fluid of MCF-7 cell.关键词
多西他赛/人乳腺癌细胞MCF-7/细胞内液/细胞外液/固相萃取-高效液相色谱法/含量测定分类
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柯昌毅,车坷科,王丽娟..固相萃取-HPLC法测定MCF-7细胞内、外液中多西他赛的含量[J].中国药房,2011,22(17):1569-1571,3.
