湖南中医药大学学报2011，Vol.31Issue(4)：8-10,3.DOI:10.3969/j.issn.1674-070X.2011.04.003.008.03
脓毒症患者CD4+CD25+调节性T细胞检测的临床意义
Clinic significance of Testing CD4+CD25+ Treg cells in patients with sepsis
邵敏 1刘宝 1王锦权 1陶晓根 1周树生 1金魁 1张翠萍2
作者信息
- 1. 安徽省立医院ICU,安徽,合肥,230001
- 2. 安徽省立医院中心实验室,安徽,合肥,230001
- 折叠
摘要
Abstract
Objective To study the level and significance of CD4+CD25+ Treg cells and CD3+、 CD4+、 CD8+ cells in peripheral blood of patients with sepsis. Methods 40 patients with sepsis Patients were divided into three groups: sepsis group(n=14), severe sepsis group(n=l5), and septic shock group (n=11).The expression of CD4+CD25+ Treg cells and CD3+、 CD4+、 CD8+ cells of 40 patients on the day 1 was detected by flow cytometry. The comparision in the expression of CD4+CD25+ Treg cells and CD3+、 CD4+、 CD8+ cells within groups was made. Twenty healthy individuals served as controls. Results The expression rate. The expression of CD4+CD25+ Treg cells was the highest in septic shock group(P＜0.05) followed by the severe sepsis group (P＜0.05) and the sepsis group the lowest(P＜0.05).The level of CD3+、 CD4+ and CD4+/CD8+in sepsis group was higher than control group and the level of CD3+、 CD4+ and CD4+/CD8+ in severe sepsis and sepsis shock was lower than control group. Conclusion The expression of CD4+CD25+ Treg cells was increased in sepsis patients and the level of CD3+、 CD4+ and CD4+/CD8+was low in severe sepsis and sepsis shock patients,suggesting that they may play an important role in pathogenesis of sepsis. Blood Treg％ level can reflect the cell immune state of patientswith sepsis and is of clinical value to assess the prognosis.关键词
脓毒症/细胞免疫/CD4+CD25+调节性T细胞Key words
Sepsis/Cell immunity/CD4+CD25+ Treg cells分类
医药卫生引用本文复制引用
邵敏,刘宝,王锦权,陶晓根,周树生,金魁,张翠萍..脓毒症患者CD4+CD25+调节性T细胞检测的临床意义[J].湖南中医药大学学报,2011,31(4):8-10,3.
