湖南中医药大学学报2011，Vol.31Issue(4)：19-21,3.DOI:10.3969/j.issn.1674-070X.2011.04.007.019.03
转染GML基因提高肺癌A549细胞对顺铂杀伤的敏感性
Transfer of GML Gene Increase the Chemosensitivity of Cisplatin to A549 Cell In Vitro
隋东江 1李伟生 1王蓉美 1陈燕侠 1王肇源 1李凤芝 1李洪霞2
作者信息
- 1. 空军总医院干部病房东楼3区,北京,100142
- 2. 吉林大学中日联谊医院中心研究室,吉林,长春,130033
- 折叠
摘要
Abstract
Objective To investigate the effect of GML gene on the chemosensitivity of cisplatin to A549 cell In vitro.Methods The GML gene was transfected stably into A549 cells by lipofectamin.The GML gene mRNA levels at A549 cell were measured by reverse transcription polymerase chain reaction (RT-PCR).The effect of GML gene on cell growth and In vitro cytotoxicity of cisplatin at various concentrations were analyzed with MTT.IC50 of cisplatin that defined as the drug concentration required to produced 50％ inhibition of cell growth was calculated for judging the chemosensitivity of drug to A549 cell.Results The RT-PCR result showed increased obviously of GML gene mRNA levels in A549 cells with transfected GML gene when compared with A549 cells without transfected GML gene.The MTT results show GML gene transfered A549 cells exhibited marked declines in proliferation when compared to the parental A549 cells.IC50 of cisplatin is also decreased four times for GML transfection A549 cells compared A549 cells.Conclusion GML gene can increase the chemosensitivity of cisplatin to A549 cells.关键词
GML基因/A549细胞/顺铂/化疗敏感性Key words
GML gene/A549 cells/Cisplatin/Chemosensitivity分类
医药卫生引用本文复制引用
隋东江,李伟生,王蓉美,陈燕侠,王肇源,李凤芝,李洪霞..转染GML基因提高肺癌A549细胞对顺铂杀伤的敏感性[J].湖南中医药大学学报,2011,31(4):19-21,3.
