中国输血杂志2011，Vol.24Issue(3)：201-205,5.
广州地区无偿献血人群HCV E1和NS5B基因的测序和分型
Sequencing and genotyping of HCV E1 and NS5B genes among volunteer blood donors in Guangzhou
摘要
Abstract
Objective To determine the sequence of HCV partial genes and the distribution of HCV genotypes among volunteer blood donors in Guangzhou. Methods A total of 201 anti-HCV( + ) plasma samples were obtained from volunteer blood donors in Guangzhou. HCV RNA was detected by fluorescent quantitation polymerase chain reaction (Q-PCR). The positive samples were tested by RT-PCR for E1 gene and NS5B gene,followed by direct DNA sequencing and phylogenetic analysis by DNASTAR,BioEdit and Mega4. 0 softwares. Results HCV RNA was positive in 109 samples out of 201 AntiHCV( + ) plasma samples. The overall positive rate of HCV RNA was 54.23％ ( 109/201 ) among anti-HCV( + ) samples,with 63.19％ (91/144)in males and 31.58％ (18/57) in females(P ＜ 0. 05 ). G enotypes were determined for the 109 HCV RNA positive samples (both based on E1 gene and NS5B gene). Among these samples,subtypes 1b,6a,3a,2a,3b,and 1a were detected at frequencies of 46.79％,33.0％,10.09％,5.50％,3.67％,and0.92％,respectively. Conclusion The positive rate of HCV RNA in males was higher than in females. And 1b and 6a were the predominant subtypes among volunteer blood donors in Guangzhou.关键词
丙型肝炎病毒(HCV)/基因测序/基因分型/HCV基因/无偿献血者/广州分类
临床医学引用本文复制引用
王敏,许茹,戎霞,夏文杰,花文峰,黄珂,罗广平,叶欣,付涌水..广州地区无偿献血人群HCV E1和NS5B基因的测序和分型[J].中国输血杂志,2011,24(3):201-205,5.基金项目
国家自然基金(30872162)、广东省自然科学基金(6021796)、广州市医药卫生重大项目(2009-ZDa-05) （30872162）
