中国输血杂志2011，Vol.24Issue(3)：206-210,5.
人外周血树突状细胞低温保存的优化研究
Study, on the optimal conditions for the cryopreservation peripheral blood derived dendritic cells
摘要
Abstract
Objective To establish a practical and efficient concentration of cryoprotective agents for the cryopreservation of dendritic cells (DCs) derived from peripheral blood.Methods Cryoprotective agents, dimethylsulfoxide( DMSO), hydroxyethyl starch (HES), human serum albumin(HSA) and fetal cow serum (FCS) were mixed in different concentrations.Group A consisted of 5％ DMSO + 6％ HES + 4％ HSA; group B 10％ DMSO + 40％ FCS; group C 12％ DMSO + 40％ FCS.Mature DCs induced from human CD14 + monocytes were cooled with each of the three groups of different cryoprotective mixtures, in a -80℃ refrigerator overnight and then the cryovials were directly transferred into the vapourphase of liquid nitrogen and stored for 24 hours.Then the DCs were thawed and cultured.The morphology, viability, phenotype, and mixed lymphocyte reaction of the cryopreserved DCs were studied and compared to those before cryopreservation.Results Compared with control mDCs, the thawed mDCs showed no distinct change in morphological characteristics, mature phenotype and SI of DCs to T cells.Conclusion Biological properties of DCs cryopreserved by uncontrolled-rate freezing technique and two-step freezing method are not changed when they are compared with fresh DCs, indicating that this method is appropriate.Group C cryopreservatives,which is 5％ DMSO + 6％ HES +4％ HAS,is more efficient for clinical application.关键词
树突状细胞/人外周血/冷冻保存/CD14+单个核细胞/混合淋巴细胞反应分类
医药卫生引用本文复制引用
李素萍,杨宏友,王震,吕蓉,郭晓婕,方勤,李敏,刘忠..人外周血树突状细胞低温保存的优化研究[J].中国输血杂志,2011,24(3):206-210,5.基金项目
安徽省国际科技合作项目(NO.07080703011) （NO.07080703011）
