中国人兽共患病学报2011，Vol.27Issue(4)：327-330,4.
新疆伊犁地区无形体流行病学调查及病原16SrRNA序列分析
Divergence of Anaplasma isolates from domestic animals in Yili region of Xinjiang Province, China
摘要
Abstract
The objective of the present study was to investigate the epidemiological situation of A. phagocytophilum in health population and animals including goats, oxen, horses, marmotas,red deer and Citellus undulatus in Yili regions, Xinjiang Province. Using micro indirect immunofluoresce assay (mIFA), IgM and IgG antibody against A. phagocytophilum were detected for sera from local healthy people and animals. Nested PCR targeted for 16SrRNA gene of A. phagocytophilum were used to test blood DNA from human and animals. Divergence of sequences of A. phagocytophilum 16SrRNA genes from domestic animals was analyzed. The positive rates of lgG antibody against to A. phagocytophilum in healthy population and goats, oxen and horses were 5.3％, 6.9％, 6.3％ and 9.1 ％ respectively. For PCR test, the potential infection rates of goats,oxen and horses were 38. 9％, 37.5％ and 36.4％ respectively. There were significant divergence of A. phogocytophilum isolates in this study and homology analysis showed that they were broadly distributed in other regions of China and other countries around China. Results indicated that infection of A. phagocytophilum in domestic animals and human exited in Yili area of Xinjiang Province. Differential diagnoses of unknown febrile patients should be emphasized in clinics and further investigation of ecological characterization of Anaplasmoses including its vectors and hosts should be practiced.关键词
无形体/流行病学调查/16SrRNA基因/新疆伊犁分类
医药卫生引用本文复制引用
范德生,禹惠兰,吴保新,王誓闻,徐琪毅,张立霞,张丽娟..新疆伊犁地区无形体流行病学调查及病原16SrRNA序列分析[J].中国人兽共患病学报,2011,27(4):327-330,4.基金项目
传染病防治科技重大专项-细菌性传染病病原谱流行规律与变异研究(2009ZX10004-203) （2009ZX10004-203）
病原体实验室网络化监测技术研究(2008ZX10004-008) （2008ZX10004-008）
国家基础研究项目-973计划2010CB530200(2010CB530206)等资助 （2010CB530206）
