中国人兽共患病学报2011，Vol.27Issue(4)：360-363,4.
ELISPOT辅助临床结核病诊断价值分析
Exploring the usage of ELISPOT as a diagnostic method for TB
摘要
Abstract
To explore the application of ELISPOT(Enzyme Linked Immunospot Assay) in the diagnosis of TB(tuberculosis) infection, TB-ELISPOT assays were performed in PBMC(peripheral blood mononuclear cell) isolated from patients who had already been clinically diagnosed as Mtb infection(54 cases) or non-TB infection(52 cases). Other diagnostic approaches,including sputum smear, sputum culture, PPD(Purified Protein Derivative Tuberculin) and DIGFA(dot immuno-gold filtration assay), were also employed for comparison of sensitivities and specificities. The data of TB-ELISPOT showed the sensitivity (Se) and specificity(Sp)were 90.7％ and 96.15 ％ respectively. The positive predictive value(PPV) and the negative predictive value(NPV) were 0.96 and 0.91 respectively. The positive likelihood ratio(+LR ) and the negative likelihood ratio(-LR) were 23.55 and 0.1 respectively. The data of DIGFA was Se 59.26％, Sp 88.46％, PPV 0.84, NPV 0.68, +LR 5.14 and -LR 0.46. The data of PPD were Se 64.81％, Sp 82.69 ％, PPV 0.80, NPV 3.74, + LR 0.69 and -LR 0.43. The data of sputum smear were Se 40.74％, Sp 100.00％, PPV1.0, NPV 0.62, +LR＞10 and -LR 0.52. The data of sputum culture was Se 25.93％, Sp 100.00％, PPV 1.0, NPV 0. 57, +LR＞10 and -LR 0.77. The sensitivity of TB-ELISPOT in sputum smear negative and sputum culture negative patients were 84.38％ and 87.5％ respectively. Results demonstrated that ELISPOT is a specific and sensitive approach in detecting Mtb active infection land latent Mtb infection. ELISPOT is a less time-consuming assay (usually 24-30 h) and could be useful in TB especially in sputum negative pulmonary tuberculosis diagnosis.关键词
结核病/诊断/酶联斑点杂交技术分类
医药卫生引用本文复制引用
朱召芹,施裕新,卢洪洲,Douglas Lowrie,卢水华,王瑶娟,熊延青,刘晓茜,万延民,席秀红,许艳,张仁芳..ELISPOT辅助临床结核病诊断价值分析[J].中国人兽共患病学报,2011,27(4):360-363,4.基金项目
上海市复旦大学青年骨干基金(07L04) （07L04）
上海市科委非政府间国际科技合作项目(10410700300) （10410700300）
"十一五"重大专项传染病防治专项课题(2008ZX10001-008) （2008ZX10001-008）
