中国实验动物学报2011，Vol.19Issue(2)：134-139,6.DOI:10.3969/j.issn.1005-4847.2011.02.011
牛卵母细胞玻璃化冷冻对基因mRNA表达量的影响
Vitrification of bovine oocytes affects their gene mRNA abundance
摘要
Abstract
Objective To evaluate the mRNA expression abundance of genes, which are related with embryonic development and ability to undergo successful fertilization, cleavage and blastocyst development, in the GV stage and in vitro matured ( IVM ) bovine oocytes. Methods The oocytes that vitrified-warmed at GV ( n = 224 ) and IVM ( n = 235 )were in vitro fertilized and cultured up to blastocyst stage under standard conditions. In parallel, we analyzed the relative abundance of Bax, Dnmtl, Hdac1, cyclinb1, Cdc2 and Cu Zn SOD transcripts of the vitrified-warmed oocytes by quantitatire real time-PCR. Result Vitrified oocytes in both GV and IVM stages showed a lower cleavage rate ( 14. 7％ ～ 89.4％,34. 5％ ～ 89.4％, P ＜ 0. 001 ) and development to blastocyst stage (3.0％ ～ 28.4％ , 12. 3％ ～ 28.4％ , P ＜ 0. 001 ) than that in the control group ( n = 238 ). In addition, vitrified oocytes in GV stage showed a lower cleavage rate ( 14. 7％ ～34. 5％ , P ＜0. 001 ) and development to blastocyst stage (3.0％ ～ 12. 3％ , P ＜0. 001 ) than those of vitrified oocytes in IVM period. In the oocytes vitrified in GV period, the Dnmtl and Cu Zn SOD expression abundance was significantly lower than that of controls ( P ＜ 0. 05 ). In the oocytes in M phase, the expression of Bax mRNA was very significantly lower than that in controls ( P ＜ 0. 01 ), and the mRNA expression of cyclinb1, Cdc2 and Cu Zn SOD was significantly lower than that of controls ( P ＜ 0. 05 ). ( P ＜ 0. 05 ). No other significant changes were found in the expression abundance of other genes.Conclusions Following vitrification the bovine oocytes in GV or IVM stage may lead to a lower transcript abundance, that may have negative effects on embryonic development.关键词
玻璃化冷冻/基因表达/牛卵母细胞/发育能力分类
生物科学引用本文复制引用
林嘉鹏,王静,赵云程,陈童,汪立芹,陈博,黄俊成..牛卵母细胞玻璃化冷冻对基因mRNA表达量的影响[J].中国实验动物学报,2011,19(2):134-139,6.基金项目
新疆自治区自然科学基金(200821182) （200821182）
新疆自治区科技攻关(200841122) （200841122）
新疆自治区科技计划项目(200711104) （200711104）
国家支撑计划(2008BADB2805-10) （2008BADB2805-10）
国家"十一五"863计划(2008aa101005). （2008aa101005）
