中国实验动物学报2011，Vol.19Issue(2)：140-144,后插8,6.DOI:10.3969/j.issn.1005-4847.2011.02.012
哺乳期接触双酚A对子代雄鼠睾丸结构及雌激素受体β表达的影响
Effects of lactational exposure to bisphenol A on the structure of testis and the expression of estrogen receptor β in adult male mouse offsprings
摘要
Abstract
Objective To study the effects of lactational exposure to bisphenol A (BPA) on the structure of testis and the expression of estrogen receptor β in adult male mouse offsprings. Methods BPA ( at a dose of 100, 50, or 5 mg/kg) was given daily by gastric garage to female mice (Mus musculus ) on postpartum days 2-22, and the mice offsprings were exposed to BPA by maternal milk. The pups were sacrificed on postnatal day (PND) 75 and the testes were removed for pathological examination, and the expression of estrogen receptor β by was assessed by immunohistochemistry, RT-PCR and Western blot analysis. Result BPA inhibited the development of testis. The appearance of the spermatogenic ceils was disordered. BPA treatment caused an up-regulation of ERβ relative expression in the testis, especially it was significantly in the low-dose group. Conclusions Lactation exposure to BPA causes morphological changes in the testis, and an up-regulation of ERβ expression in the testis of the male offsprings, leading to changes in the development of testis.关键词
双酚A/哺乳期/睾丸/雌激素受体β分类
生物科学引用本文复制引用
丁保清,张义军,解美娜,李莎莎..哺乳期接触双酚A对子代雄鼠睾丸结构及雌激素受体β表达的影响[J].中国实验动物学报,2011,19(2):140-144,后插8,6.基金项目
山东省高等学校科技计划项目(No.J08LG77). （No.J08LG77）
