首页|期刊导航|中华医学杂志（英文版）|Drug synergistic antifertility effect of combined administration of low-dose gossypol with steroid hormones in rats
中华医学杂志（英文版）2011，Vol.124Issue(11)：1678-1682,5.DOI:10.3760/CMA.J.ISSN.0366-6999.2011.11.014
Drug synergistic antifertility effect of combined administration of low-dose gossypol with steroid hormones in rats
Drug synergistic antifertility effect of combined administration of low-dose gossypol with steroid hormones in rats
摘要
Abstract
Background Our previous studies suggested that low-dose gossypol combined with steroid hormones has a reversible antifertility role in adult male rats, and the course of treatment was shorter than that of either gossypol or steroid hormones alone. This result suggested that low-dose gossypol and steroid hormones have a drug synergistic effect on antifertility. The aim of the study was to find the target organs of the antifertility synergistic effect of the combined regimen.Methods Thirty-two adult male rats were divided into four groups randomly: group GH, rats were fed orally with group H, the same dosage of DSG/EE/TU as in group GH were administered; group C, rats were treated with vehicle (1% methyl cellulose) as control. Testes and epididymis were removed at 8 weeks post-treatment for evaluating their weight,volumes, volume fraction, and total volume of testicular tissue structures and the seminiferous tubule diameter using stereological assay. Sperm cell numbers and the motility of epididymal sperm were quantitated by flow cytometry and morphological methods.Results Compared with group C, spermatogenesis was normal in group G and suppressed in groups H and GH.Similar changes of testicular tissue structures and sperm number were found in groups H and GH. The decreases of epididymal sperm number and motility in group GH were greater than that of the low-dose gossypol or steroid hormones alone group.Conclusions The suppression of spermatogenesis was induced by steroid hormones in the combined regimen, and the epididymis was the target organ of low-dose gossypol. Combined use of low-dose gossypol and steroid hormones played a comprehensive antifertility role in their synergistic effect on reducing the number and motility of epididymal sperm.关键词
gossypol/steroid hormone/antifertility/synergistic effect/ratKey words
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CHANG Qing,LIU Zhe,MA Wen-zhi,HEI Chang-chun,SHEN Xin-sheng,QIAN Xiao-jing,XU Zeng-lu..Drug synergistic antifertility effect of combined administration of low-dose gossypol with steroid hormones in rats[J].中华医学杂志（英文版）,2011,124(11):1678-1682,5.基金项目
This work was supported by the grants from Natural Science Foundation of Ningxia,China (No.NZ10104 and No.NZ1080). （No.NZ10104 and No.NZ1080）
