南京医科大学学报（自然科学版）2011，Vol.31Issue(4)：491-494,4.
新型KATP开放剂埃他卡林对原代培养人肺动脉平滑肌细胞PCNAmRNA表达的影响
Effect of iptakalim,a novel KATP opener,on the expression of proliferating cell nuclear antigen mRNA in primary cultured human pulmonary artery smooth muscle cells
摘要
Abstract
Objective: To investigate the effect of iptakalim(IPT),a novel ATP sensitive potassium(KATP) opener,on the expression of proliferating cell nuclear antigen (PCNA) mRNA in primary cultured human pulmonary artery smooth muscle cells. Methods:Under germfree conditions,the smooth muscular layers were separated from human pulmonary arterioles(3rd～4th divisions). Human pulmonary artery smooth muscle cells were cultured by tissue block method and were divided into following groups:control group, hypoxia group,hypoxia+iptakalim or pinacidil group and hypoxia+iptakalim or pinacidil+glibenclimide group. Fluorescence quantitative reverse transcription-polymerase chain reaction (FQ-RT-PCR)was used to measure the expression level of PCNA mRNA. Results:The expression of PCNA mRNA in hypoxia group increased(3.90±0.24) times higher than that in control group. IPT at the concentrations of 1O-7, 10-6 and 10-5 mol/L lowered the expression of PCNA mRNA by (26.00±2.01)％, (55.33±4.04)％, (79.00±1.00)％ ,respectively, compared with that in hypoxia group(P ＜ 0.01 ). Glibenchmide (GLI) at the concentration of 10-5 mol/L antagonized the effect of IPT with the same concentration on the expression of PCNA mRNA,compared with that in hypoxia + IPT 10-5 mol/L group (P ＜ 0.01 ).Conclusion:The expression of PCNA mRNA in human pulmonary artery smooth muscle cells under hypoxic conditions increased,while IPT has an inhibitory effect on the expression of PCNA mRNA in a concentration-dependent manner through activating KATP channels.关键词
ATP敏感性钾通道/埃他卡林/增殖细胞核抗原/人肺动脉平滑肌细胞Key words
ATP sensitive potassium channel/iptakalim/proliferating cell nuclear antigen (PCNA)/human pulmonary arterial smooth muscle cell分类
医药卫生引用本文复制引用
周燕娟,解卫平,张石江,李庆玲,朱煜明,王虹..新型KATP开放剂埃他卡林对原代培养人肺动脉平滑肌细胞PCNAmRNA表达的影响[J].南京医科大学学报（自然科学版）,2011,31(4):491-494,4.基金项目
江苏省自然科学基金资助(BK2006246) （BK2006246）
