重庆医学2011，Vol.40Issue(16)：1616-1618,3.DOI:10.3969/j.issn.1671-8348.2011.16.023
乌司他丁联合甲基泼尼松龙对大鼠肺缺血再灌注损伤的保护作用
Protective effects of ulinastatin combined with methylprednisolone on pulmonary ischemia reperfusion injury in rats
郭亮 1唐忠志 1程青 1邬明 1曾尟枚 1韩璇 1杨李2
作者信息
- 1. 广州军区武汉总医院,急诊科,湖北武汉,430070
- 2. 广州军区武汉总医院,医学实验科,湖北武汉,430070
- 折叠
摘要
Abstract
Objective To explore the influence of ulinastatin combined with methylprednisolone on aquaporin l(AQP 1) and tumor necrosis factor-alpha(TNF-α) in serum when ischemia reperfusion injury occured in rats. Methods 40 Wistar rats were ran domly devided into 5 groups(n =8):normal control group, model control group, methylprednisolone group(30 mg/kg), ulinastatin group(50 000 U/kg) and combination group[ulinastatin(50 000 U/kg) combined with methylperdnisolone(30 mg/kg)]. Levels of AQP l and TNF-α in serum were detected by enzyme linked immunosorbent assay(ELISA) ;Protein contents in bronchoalveolar lavage fluid were assayed by Commassie blue staining; Wet and dry mass were measured by scale and pulmonary morphological changes were observed by optical microscope. Results Compared with model control group, serum concentration of AQP l was significantly increased and that of TNF-α decreased in ulinastatin group and methylprednisolone group, especially in combination group(P＜0.01) ;Protein contents in bronchoalveolar lavage fluid and wet/dry mass ratios were both markedly reduced in ulinastatin,methylprednisolone and combination groups(P＜0.01). Conclusion Ulinastatin combined with methylprednisolone administration has better therapeutic effects on acute lung injury caused by ischemia reperfusion in rats, which mechanism probably correlated with increasing level of serum AQP l and decreasing that of TNF-α.关键词
再灌注损伤,肺/乌司他丁/甲基泼尼松龙/水通道蛋白/肿瘤坏死因子αKey words
reperfusion injury/ pulmonary/ ulinastatin/ methylprednisolone/ aquaporin 1/ tumor necrosis factor-alpha引用本文复制引用
郭亮,唐忠志,程青,邬明,曾尟枚,韩璇,杨李..乌司他丁联合甲基泼尼松龙对大鼠肺缺血再灌注损伤的保护作用[J].重庆医学,2011,40(16):1616-1618,3.
