应用生态学报2011，Vol.22Issue(4)：929-935,7.
内蒙古呼伦贝尔草原土壤氨氧化细菌多样性及群落结构
Diversity and community structure of soil ammonia-oxidizing bacteria in Hulunbeier Grassland,Inner Mongolia
摘要
Abstract
By the methods of polymerase chain reaction-denaturing gradient gel electrophoresis and sequence analysis, a comparative study was conducted on the diversity and community structure of soil ammonia-oxidizing bacteria in the Filifolium sibiricum steppe , Stipa baicalensis steppe , Leymus chinensis steppe, Stipa grandis steppe, and Stipa kryrowi steppe in Hulunbeier Grassland. Inner Mongolia. A significant difference was observed in the community structure of soil ammonia-oxidizing bacteria among the five steppes, with the similarity lower than 50％ . The diversity of soil ammonia-oxidizing bacteria was the highest in F. sibiricum steppe, followed by in S. baicalensis steppe, L. chinensis steppe, S. kryrowi steppe, and S. grandis steppe. In the five steppes, Nitrosospira cluster 3 was the dominant group, and the Nitrosospira cluster 1. 2. and 4 as well as Nitrosomonas were also found. The community structure of soil ammoma oxidizing bacteria in F. sibiricum steppe was most complex, while that in L. chinensis steppe and S. grandis steppe was relatively simple. Correlation analysis indicated that there existed significant positive correlations between the diversity of soil ammonia-oxidizing bacteria and the soil moisture. total nitrogen, total organic carbon. and C/N ratio ( P＜0.05 ) .关键词
呼伦贝尔草原/氨氧化细菌/多样性/系统发育分析分类
生物科学引用本文复制引用
文都日乐,李刚,杨殿林,张静妮,易津..内蒙古呼伦贝尔草原土壤氨氧化细菌多样性及群落结构[J].应用生态学报,2011,22(4):929-935,7.基金项目
国家自然科学基金项目(31000242,30770367)和中国农业科学院院长基金项目资助. （31000242,30770367）
