江西农业学报2011，Vol.23Issue(3)：14-16,19,4.
芦笋游离小孢子培养研究初报
A Preliminary Study on Isolated Microspore Culture of Asparagus officinalis
摘要
Abstract
In this paper, the isolated microspore culture is studied with five cultivars or hybrid combinations of Asparagus officinalis. The results show that the optimal time for the inducement of callus is near the monokaryotic stage; there is significant difference in the inducement rate of callus among different genotypes; the frequency of the inflated microspore treated by different temperatures is 6 times higher than that of the control, and the highest frequency reaches 22.5％; the suitable medium for the isolated microspore culture is MS + 0.5 mg/L 6 - BA + 1.0 mg/L NAA + 0.2 mg/L 2,4 - D + 400 mg/L Glu + 6％ sucrose.关键词
芦笋/小孢子培养/愈伤组织/胚状体分类
农业科技引用本文复制引用
汤泳萍,周劲松,罗绍春,盛文涛,谢启鑫,朱友林,陈光宇..芦笋游离小孢子培养研究初报[J].江西农业学报,2011,23(3):14-16,19,4.基金项目
公益性行业(农业)科研专项"芦笋产业技术研究与试验示范"(201003074) （农业）
江西省农科院青年创新基金"芦笋小孢子培养技术研究". （）
