江西农业学报2011，Vol.23Issue(3)：126-128,131,4.
黄蜀葵花器官中β-半乳糖苷酶的提取纯化和酶学特性研究
Purification of β-galactosidase from Flowers of Abelmoschus manihot and Its Characteristics
摘要
Abstract
In this paper, the orthogonal experiment was used to optimize the condition for the extraction of β -galactosidase from the flowers of Abelmoschus manihot by taking the total activity of the enzyme as index, the saturation of ammonium sulfate in saltingout method was determined based on the enzyme activity and protein content, and the optimum pH -value and temperature as well as the thermal stability for the extracted β - galactosidase were studied by using ONPG as the substrate. The results indicated that the optimum condition of extracting β - galactosidase from the flowers of Abelmoschus manihot was extracting 12 h in 3 - times volume ( V/ W) of phosphate buffer at 4 ℃. The optimum saturation of ammonium sulfate for salting - out was 30％ and 70％. The β - galactosidase reached the highest total activity and had better thermal stability at 55 ℃, and its activity decreased rapidly above 70 ℃.关键词
黄蜀葵/β-半乳糖苷酶/提取/纯化分类
农业科技引用本文复制引用
王雅男,王康才,李同根,饶琳莉,陈慧..黄蜀葵花器官中β-半乳糖苷酶的提取纯化和酶学特性研究[J].江西农业学报,2011,23(3):126-128,131,4.基金项目
江苏苏中药业项目 （）
南京农业大学SRT项目. （）
